Product nameAnti-HIRA antibody
See all HIRA primary antibodies
DescriptionRabbit polyclonal to HIRA
Specificityab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA and a band at 89kDa, corresponding to the short isoform of HIRA in HepG2 nuclear extract.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Dog
- This antibody gave a positive signal in the following whole cell lysate: HepG2 nuclear extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab20655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA.
FunctionCooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificityExpressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
Sequence similaritiesBelongs to the WD repeat HIR1 family.
Contains 8 WD repeats.
Developmental stageExpressed during embryogenesis.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
Cellular localizationNucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
- Information by UniProt
- DGCR1 antibody
- DiGeorge critical region gene 1 antibody
- HIR antibody
ab20655 at 10 µg + HepG2 nuclear extract lysate at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 89 kDa (possible isoform)
ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA and a band at 89kDa, corresponding to the short isoform of HIRA
This product has been referenced in:
- Rivera C et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res 43:9097-106 (2015). Read more (PubMed: 26405197) »
- Majumder A et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). Read more (PubMed: 25847244) »