Anti-HIRA/HIR antibody (ab20655)
Key features and details
- Rabbit polyclonal to HIRA/HIR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-HIRA/HIR antibody
See all HIRA/HIR primary antibodies -
Description
Rabbit polyclonal to HIRA/HIR -
Host species
Rabbit -
Specificity
ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR in HepG2 nuclear extract.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR. |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR. |
Target
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Function
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. -
Tissue specificity
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. -
Sequence similarities
Belongs to the WD repeat HIR1 family.
Contains 8 WD repeats. -
Developmental stage
Expressed during embryogenesis. -
Post-translational
modificationsSumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. -
Cellular localization
Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. - Information by UniProt
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Database links
- Entrez Gene: 7290 Human
- Entrez Gene: 15260 Mouse
- Entrez Gene: 363849 Rat
- Omim: 600237 Human
- SwissProt: P54198 Human
- SwissProt: Q61666 Mouse
- Unigene: 474206 Human
- Unigene: 15694 Mouse
see all -
Alternative names
- DGCR1 antibody
- DiGeorge critical region gene 1 antibody
- HIR antibody
see all
Images
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ab20655 at 10 µg + HepG2 nuclear extract lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 89 kDa (possible isoform)ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab20655 has been referenced in 10 publications.
- Hormazabal J et al. Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control. Nucleic Acids Res 50:1875-1887 (2022). PubMed: 35037039
- Locatelli M et al. HIRA Supports Hepatitis B Virus Minichromosome Establishment and Transcriptional Activity in Infected Hepatocytes. Cell Mol Gastroenterol Hepatol 14:527-551 (2022). PubMed: 35643233
- Saavedra F et al. JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability. Epigenetics Chromatin 13:6 (2020). PubMed: 32070414
- Zhang Q et al. Role of lncRNA uc.457 in the differentiation and maturation of cardiomyocytes. Mol Med Rep 19:4927-4934 (2019). PubMed: 30957182
- Cohen C et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
- Rivera C et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res 43:9097-106 (2015). PubMed: 26405197
- Majumder A et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). PubMed: 25847244
- O'Sullivan RJ et al. Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1. Nat Struct Mol Biol 21:167-74 (2014). WB ; Human . PubMed: 24413054
- Lewis PW et al. Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres. Proc Natl Acad Sci U S A 107:14075-80 (2010). ChIP ; Mouse . PubMed: 20651253
- Dutta D et al. Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem 285:41567-77 (2010). WB ; Mouse . PubMed: 21041298