Anti-Hirudin antibody [2D7] (ab17198)
Key features and details
- Mouse monoclonal [2D7] to Hirudin
- Suitable for: ELISA, WB
- Reacts with: Leech
- Isotype: IgG1
Overview
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Product name
Anti-Hirudin antibody [2D7] -
Description
Mouse monoclonal [2D7] to Hirudin -
Host species
Mouse -
Specificity
The affinity (Kd) of this antibody for rHV2K47 is 4.5x10E-7 M -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Leech -
Immunogen
Recombinant full length protein corresponding to Leech Hirudin.
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Epitope
The epitope of this antibody is located at the C-terminal end of hirudin. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D7 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/2000.
|
|
WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 7 kDa.
ab17198 reacts with hirudin in both reduced and unreduced forms. |
Notes |
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ELISA
1/2000. |
WB
Use at an assay dependent concentration. Detects a band of approximately 7 kDa. ab17198 reacts with hirudin in both reduced and unreduced forms. |
Target
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Relevance
Hirudin is a highly effective and very specific anticoagulant which specifically inhibits formation of fibrin and activation of factors V, VIII and XIII by forming irreversible, noncovalent 1:1 complexes with a-thrombin. Hirudin is a 65 amino acid polypeptide, 7 kDa protein naturally originating from the medical leech Hirudo medicinalis. It is the most potent thrombin inhibitor known to date. -
Cellular localization
Secreted -
Database links
- SwissProt: P01050 Leech
- SwissProt: P28506 Leech
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Alternative names
- Hirudin 1 antibody
- Lepirudin antibody
- Refludan antibody
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab17198 has been referenced in 1 publication.
- Wüstenhagen DA et al. Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis. Sci Rep 10:19818 (2020). PubMed: 33188246