Product nameHis-Tag Protein Expression Check Kit
His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA. The assay provides a qualitative yes/no answer, although a semiquantitative measurement of the protein abundance in the sample can be determined using internal references and controls.
This product is manufactured by Expedeon, an Abcam company. It was previously called His-Tag Check&Go!. Expedeon product code 4003-0030 is the same as the 30 test size of this product.
A His-tagged protein on the “Test line” (T line) and a ULFA-tagged protein on the “Control line” (C line) are immobilized on the nitrocellulose strips.
40nm Gold conjugated anti-HisTag antibody and 40nm Gold conjugated anti-ULFA tag conjugates are dried on the conjugate pad and will selectively bind to the T and C line, respectively. The C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.
His-Tag Protein Expression Check Kit is formulated as a competitive assay so that when the sample does not contain any His-tagged protein, the T line will appear as a strong red line. On the contrary, the T line will not be visible (or appear as a pale red line) if the sample contains the His- tagged protein that competes with the binding of the Gold conjugated anti-HisTag antibody conjugate to the antigen immobilized on the T line.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 30 tests 10X Running Buffer 1 vial His-Tag Protein Expression Check Strips 1 x 30 tests
Six different recombinant proteins were diluted down to 300, 100, 33 and 11ug/mL in 1X Running Buffer and run on a His-Tag Protein Expression Check strip. The strips were analysed using the EseQuant reader. Signal suppression is clearly detected in all the proteins tested.
Non induced (-I) and induced (+I) E. coli cell lysates expressing recombinant protein His-GST in 1X Running Buffer
ab270048 has not yet been referenced specifically in any publications.