Product nameHis-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap
His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.
The Ni-NTA resin contained in the column consists of 90μm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives.
This product is manufactured by Expedeon, an Abcam company. It was previously called AminTRAP HIS Prepacked Columns 5ml - Pack of 5. Expedeon product code TNN50005 is the same as the 5 unit size of this product.
The column has a standard interface and can be adapted to all kinds of chromatography systems.
- Compatible with ÄKTA systems
- Column size: 1.6 x 2.5cm.
- Binding capacity: >40mg His-tagged protein/ml medium
- Particle size: 45-165µm.
- Max pressure: 0.3Mpa, 3 bar.
Storage instructionsShipped at Room Temperature. Store at +4°C. Please see notes section.
Storage bufferpH: 6
Constituents: PBS, 20% Ethanol
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ab270529 has not yet been referenced specifically in any publications.