Product nameHis-tagged Protein Gold Detection Kit
His-tagged Protein Gold Detection Kit (ab170734) is intended and optimized for direct visualization of recombinant His-tagged proteins and other proteins bearing different histidine tags in western or dot blotting applications. Unlike detection by anti-6xHis antibodies, His-tagged Protein Gold Detection does not require a specific location of the polyhistidine tag (N- or C-terminus), does not need primary and secondary antibody incubations, or the presence of specific adjacent amino acid sequences.
His-tagged Protein Gold Detection Kit uses Nickel-NTA (nickel-nitrilotriacetic acid)-functionalized gold nanoparticles to specifically bind to His-tagged proteins. With the autometallographic amplification subsequently applied to the gold nanoparticles, His-tagged Protein Gold Detection allows the direct visualization of His-tagged proteins. His-tagged Protein Gold Detection generates specific purple colored metallic bands or dots which do not fade and will not dissolve in water and organic solvents. The His-tagged Protein Gold Detection Kit can detect nanogram levels of purified His-tagged proteins. The entire procedure takes about 1 hour.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit AutoMet Detect A 1 x 40ml AutoMet Detect B 1 x 40ml AutoMet Detect C 1 x 40ml AutoMet Detect D 1 x 40ml Nickel-NTA-Gold 1 x 1.5ml
ab170734 has not yet been referenced specifically in any publications.