Overview

  • Product name
    His-tagged Protein Gold Detection Kit
  • Product overview

    His-tagged Protein Gold Detection Kit (ab170734) is intended and optimized for direct visualization of recombinant His-tagged proteins and other proteins bearing different histidine tags in western or dot blotting applications. Unlike detection by anti-6xHis antibodies, His-tagged Protein Gold Detection does not require a specific location of the polyhistidine tag (N- or C-terminus), does not need primary and secondary antibody incubations, or the presence of specific adjacent amino acid sequences.

    His-tagged Protein Gold Detection Kit uses Nickel-NTA (nickel-nitrilotriacetic acid)-functionalized gold nanoparticles to specifically bind to His-tagged proteins. With the autometallographic amplification subsequently applied to the gold nanoparticles, His-tagged Protein Gold Detection allows the direct visualization of His-tagged proteins. His-tagged Protein Gold Detection generates specific purple colored metallic bands or dots which do not fade and will not dissolve in water and organic solvents. The His-tagged Protein Gold Detection Kit can detect nanogram levels of purified His-tagged proteins. The entire procedure takes about 1 hour.

Properties

Protocols

References

ab170734 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170734.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up