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    histamine-assay-kit-colorimetric-ab235630.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Histamine Assay Kit (Colorimetric) (ab235630)

  • Datasheet
  • Protocol Booklet
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Example Data
  • Estimation of Histamine concentration in putrid fish and fish sauce.

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader

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Overview

  • Product name

    Histamine Assay Kit (Colorimetric)
    See all Histamine kits
  • Detection method

    Colorimetric
  • Assay type

    Quantitative
  • Product overview

    Histamine Assay Kit (Colorimetric) (ab235630) can be used to identify dangerous levels of histamine in various fish and meat products, as well as other fermented food and sauces/beverages. While the body is accustomed to exposure to low levels (30-50 ppm, or mg/L) of histamine, ingestion of food containing levels higher than several hundred parts per million can lead to adverse effects.


    With this kit histamine can be detected in samples at concentrations as low as 10 μM, or 10 μg/mL (10 ppm). This kit is very specific and does not interact with other bioamines.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K506 Histamine Assay Kit (Colorimetric). K506-100 is the same size as the 100 test size of ab235630.

    Histamine is a biogenic amine with considerable biological relevance. As a signaling molecule histamine plays manifold roles in the body, ranging from local immune responses to neurotransmission. Histamine is also frequently encountered in food, particularly fish and fermented food products such as sauerkraut and aged cheeses. This is because some bacteria generate histamine from histidine via the enzyme histidine decarboxylase. Elevated levels of bacterial fermentation can lead to elevated histamine in raw meat and food products. Histamine levels thus can be used as an indicator of spoilage.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Histamine Enzyme Mix 1 vial
    Histamine Probe 1 vial
    Histamine Standard (50 mM) 1 x 100µl
    Histamine Assay Buffer 1 x 25ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
  • Relevance

    Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.
  • Cellular localization

    Secreted
  • Alternative names

    • 2 (4 imidazolyl)ethylamine
    • 4 (2 aminoethyl) 1 3 diazole

Associated products

  • Related Products

    • 10kD Spin Column (ab93349)

Images

  • Example Data
    Example Data

    Histamine Standard Curve.

  • Estimation of Histamine concentration in putrid fish and fish sauce.
    Estimation of Histamine concentration in putrid fish and fish sauce.

    Fish sauce was deproteinized using 10 kDa spin colum and diluted 20-fold using Histamine Assay Buffer. Histamine concentrations are: Putrid Fish: 88 ± 8 ppm, Fish Sauce: 847 ± 27 ppm.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab235630? Please let us know so that we can cite the reference in this datasheet.

ab235630 has not yet been referenced specifically in any publications.

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