Key features and details
- Sensitivity: 0.03 ng/ml
- Range: 0.098 ng/ml - 25 ng/ml
- Sample type: Cell culture media, EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Dog, Human
Product nameHistamine ELISA kit
See all Histamine kits
Intra-assay Sample n Mean SD CV% 1 20 4.62ng/ml 7.1% 2 20 0.89ng/ml 4.9% 3 20 0.23ng/ml 8.4% Inter-assay Sample n Mean SD CV% 1 4.73ng/ml 8.9% 2 0.94ng/ml 4.8% 3 0.26ng/ml 7.9%
Sample typeUrine, Serum, Cell culture media, EDTA Plasma
Range0.098 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Urine 96.97 71.9% - 115% Serum 121 111% - 131% Cell culture media 82 44% - 102% EDTA Plasma 123.33 109% - 148%
Assay time2h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse, Rat, Dog, Human
Histamine ELISA Kit (ab213975) is a competitive ELISA kit for the quantitative determination of histamine in human, mouse, rat and canine sample matrices. This competitive, ultra-sensitive ELISA does not require acylation of histamine and has a time-to-answer of 2 hours. Please read the complete kit insert before performing this assay.
In this assay samples or standards are added to wells coated with a goat anti-rabbit IgG antibody. A polyclonal antibody to histamine and a solution of biotinylated histamine tracer are both added to the wells and the plate is incubated. During this incubation, the antibody binds the histamine in the sample or tracer in a competitive manner. The plate is washed, leaving only bound histamine on the plate. A solution of Horseradish Peroxidase conjugated Streptavidin (SA-HRP) is added to all wells and the plate is incubated. The plate is washed to remove excess conjugate. TMB substrate is added to the wells and the plate is incubated. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450nm. The amount of signal is inversely proportional to the level of Histamine in the sample.
Neat (undiluted) methanol-extracted human serum and plasma samples, neat urine and tissue culture media in addition to mouse, rat and canine samples have been validated for use in this. However, due to variation in samples, dilution may be required. For detailed methanol-extraction method, please refer to Protocol Booklet.
Histamine is an endogenous short-acting biogenic amine synthesized from the amino acid histidine and is widely distributed throughout the body. Histamine is an important mediator of immediate-type-allergic reactions. Normally, there is a minimal amount of histamine circulating in the human body. An allergic reaction can occur when a substance enters the body (i.e. food, chemicals, insect bites, oils from plants). In response to the molecule, the body will then release larger quantities of histamine, which starts a cascade of events and begins an immune response. The body balances the histamine release by producing Epinephrine (adrenaline) which can help modulate the effects of histamine. When histamine is released, part of the cascade of events is inflammation. Antihistamines work by blocking the action of histamine and the resulting inflammation, amongst other symptoms, to provide relief.
Anaphylaxis occurs when there is a hypersensitive response. Reactions can range from mild to severe and, in extreme cases, can be fatal. Symptoms of hypersensitivity to histamine include hives, tingling feeling in the mouth, difficulty breathing, stomach cramping and diarrhea.
Elevations in plasma and tissue histamine levels have been reported during anaphylaxis and experimental systems investigating the allergic responses of the skin and airways. Because of its potent role in the immune response, histamine in the body is present in a very transitory state and can only be measured within minutes of release. The half-life of histamine in a biological system is four minutes before conversion to n-methyl histamine. N-methyl histamine is a major product of histamine metabolism, is present in urine and has a longer half-life than that of histamine. This assay reacts equally to N-methyl histamine and histamine, making it an excellent choice for measuring histamine levels in urine.
Histamine is also in a class of neurotransmitters called “Small Molecule Neurotransmitter Substances”. This group includes molecules such as Serotonin, Epinephrine and Dopamine. Additionally, histamine plays a role in gastric acid secretion, assisting in the induction of acid production.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests (20X) Wash Buffer 1 x 100ml Antibody Diluent 1 x 6ml Assay Buffer 1 x 25ml Goat anti-Rabbit IgG coated microplate (12x 8 well strips) 1 unit Histamine Antibody (Lyophilized) 1 vial Histamine Standard Stock (250 ng/mL) 1 x 200µl Histamine Tracer (Lyophilized) 1 vial Plate Sealer 3 units SA-HRP Conjugate 1 x 20ml Stop Solution (1N Soln.) 1 x 10ml TMB Substrate 1 x 25ml Tracer Diluent 1 x 6ml
RelevanceHistamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.
- 2 (4 imidazolyl)ethylamine
- 4 (2 aminoethyl) 1 3 diazole
ab213975 has been referenced in 9 publications.
- Van Nguyen T et al. Anti-allergic rhinitis activity of a-lipoic acid via balancing Th17/Treg expression and enhancing Nrf2/HO-1 pathway signaling. Sci Rep 10:12528 (2020). PubMed: 32719431
- Lei H et al. IL-37 relieves allergic inflammation by inhibiting the CCL11 signaling pathway in a mouse model of allergic rhinitis. Exp Ther Med 20:3114-3121 (2020). PubMed: 32855679
- Hu B et al. Nuclear factor E2 related factor (NRF2) inhibits mast cell- mediated allergic inflammation via SIRT4-mediated mitochondrial metabolism. Ann Palliat Med 9:3839-3847 (2020). PubMed: 33222465
- Nian JB et al. Epithelial cells expressed IL-33 to promote degranulation of mast cells through inhibition on ST2/PI3K/mTOR-mediated autophagy in allergic rhinitis. Cell Cycle 19:1132-1142 (2020). PubMed: 32298206
- Almishri W et al. The Antidepressant Mirtazapine Inhibits Hepatic Innate Immune Networks to Attenuate Immune-Mediated Liver Injury in Mice. Front Immunol 10:803 (2019). PubMed: 31031775
- Klein O et al. Mammalian diaphanous-related formin 1 (mDia1) coordinates mast cell migration and secretion through its actin-nucleating activity. J Allergy Clin Immunol N/A:N/A (2019). PubMed: 31279009
- Guo C & Chang TMS Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly 5% blood volume top-loading followed by a challenge of 30% exchange transfusion. Artif Cells Nanomed Biotechnol 46:1349-1363 (2018). PubMed: 29873524
- Li L et al. MicroRNA-let-7e regulates the progression and development of allergic rhinitis by targeting suppressor of cytokine signaling 4 and activating Janus kinase 1/signal transducer and activator of transcription 3 pathway. Exp Ther Med 15:3523-3529 (2018). PubMed: 29545878
- Shirbin SJ et al. Architectural Effects of Star-Shaped "Structurally Nanoengineered Antimicrobial Peptide Polymers" (SNAPPs) on Their Biological Activity. Adv Healthc Mater 7:e1800627 (2018). PubMed: 30175464