Histamine ELISA Kit (ab285333)
Key features and details
- Sensitivity: 0.938 ng/ml
- Range: 1.563 ng/ml - 100 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Histamine ELISA Kit
See all Histamine kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Other biological fluids, Tissue Homogenate -
Assay type
Competitive -
Sensitivity
< 0.938 ng/ml -
Range
1.563 ng/ml - 100 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
This ELISA kit is used for in vitro quantitative determination of Histamine.
Species reactivity: Universal.
Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine is involved in the inflammatory response and has a central role as a mediator of pruritus. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
This Histamine ELISA kit (ab285333, K4163) uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with histamine. During the reaction, histamine in the sample or standard competes with a fixed amount of histamine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to histamine. The concentration of histamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K4163 Histamine (HIS) ELISA Kit. K4163-100 is the same size as the 96 test size of ab285333.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin-Detection antibody (Lyophilized) 1 vial HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 2 vials Micro ELISA Plate 1 unit Plate Sealer 5 units Purified water 1 x 200µl SABC dilution buffer 1 x 10ml Sample and Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Relevance
Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. -
Cellular localization
Secreted -
Alternative names
- 2 (4 imidazolyl)ethylamine
- 4 (2 aminoethyl) 1 3 diazole
Images
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Histamine ELISA Kit Standard Curve
Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay
Datasheets and documents
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Datasheet download
References (0)
ab285333 has not yet been referenced specifically in any publications.
