• Product name
    Anti-Histidine decarboxylase antibody
    See all Histidine decarboxylase primary antibodies
  • Description
    Rabbit polyclonal to Histidine decarboxylase
  • Host species
  • Specificity
    Detects both the 74 kDa full length and the 54 kDa active form of Histidine decarboxylase.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Histidine decarboxylase. synthetic peptide around aa 22
    (Peptide available as ab55801)



Our Abpromise guarantee covers the use of ab37291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21873469
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Histidine decarboxylase peptide (ab55801).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.



  • Western blot analysis of HDC expression in rat fetal liver tissue lysate.
  • IHC image of ab37291 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37291, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Chen J  et al. Aggravated myocardial infarction-induced cardiac remodeling and heart failure in histamine-deficient mice. Sci Rep 7:44007 (2017). WB . Read more (PubMed: 28272448) »
  • Nizamutdinova IT  et al. Involvement of histamine in endothelium-dependent relaxation of mesenteric lymphatic vessels. Microcirculation 21:640-8 (2014). Read more (PubMed: 24750494) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for stopping by our booth at SfN and for your interest in our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications a...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.
Please keep me updated about the results from repeating the staining, and if the problem persists then I would be happy to send replacements f...

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Thank you for calling Abcam earlier today.

I am sorry that you were having some problems using ab37291 in western blot. As we discussed on the telephone, I am sending you a new vial of this this to try out. Your new order number is ********. ...

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Thank you for your reply. I am happy to offer you a replacement product. I can arrange this through the distributor - please provide the distributor name. Also please indicate if you are okay to receive another vial of this antibody or a credit. ...

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Thank you for your reply. In order to send a replacement I will need a specific order number. Did you order the item directly from Abcam or through a distributor? I look forward to your reply so that I may assist you further.

Thank you for your reply. I can see that my colleagues are assisting you with ab3611 and ab15323. The protocol suggestions that I have given you are specific for ab37291 and I see that they have also offered protocol suggestions for these products. ...

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Thank you for contacting Abcam regarding ab37291. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to improve your resu...

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