Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)


  • Product name
    Histone Acetyltransferase Activity Assay Kit (Colorimetric)
    See all Histone acetyltransferase kits
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.


Associated products


  • HAT activity in NIH3T3 cells, which were transfected with the desired plasmids by using Lipofectamine 2000. After 24 hours from transfection, the nuclear protein was extracted and the Histone acetyltransferase activity was measured by using ab65352. 

  • Histone acetylation activity assay for L.donovani cells over-expressing HAT1, HAT2, HAT3 and HAT4. The activities were compared to wild-type (WT) cells and pLPneo2 (vector) only transfected cells. The relative activity was determined using Histone Acetyltransferase acitvity assay kit (ab65352). 

  • Analysis of Histone Acetyltransferase Activity in HeLa Nuclear Extract using ab65352. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a micro plate reader at 440 nm according to the kit instructions.



This product has been referenced in:
  • Jha PK  et al. HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani. PLoS One 12:e0177372 (2017). Leishmania . Read more (PubMed: 28486547) »
  • Goriki A  et al. A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. PLoS Biol 12:e1001839 (2014). Read more (PubMed: 24736997) »

See all 11 Publications for this product

Customer reviews and Q&As

Rat liver nuclear fraction

Excellent Excellent 5/5 (Ease of Use)
Our samples were not within the linear range for the booklet's recommended protein concentration. A preliminary serial dilution curve should be prepared prior to running the full assay so as to ensure OD values are within the linear range.

Abcam user community

Verified customer

Submitted Apr 01 2015

You can use a background control sample, also use a nuclear extract.

I have had the chance to inquire to the lab about the use of a purified IP sample. Theywould suggest using the initial sample without any IP for the HAT assay. If the IP is just for concentrating the sample, they recommend use of spin columns, not IP. ...

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The lab has confirmed that this kit has not been validated for measuring Tubulin Acetyltransferase activity.  We have no information about whether it would be suitable for this purpose, but the kit was specifically designed for measuring Histone Acetyl...

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Thank you very much for your inquiry. 1.) I can confirm that the HAT activity can be expressed as the relative O.D. value per µg or nmol/min/µg sample. Please have a look at the following example calculation: A. Relative OD/ug: One obtains...

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DDT should be avoided, as this compound strongly interferes with the reactions in the kit. There are a few things I would like to suggest: - After acid extracting the histones in your current protocol, the sample could be dialysed overnight in 1...

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As long as the tissue samples were frozen appropriately, nuclear lysates generated from these tissues can be used for the HAT assay.

The feedback from the lab is that technically BME should be fine but we have not tested this specifically.

Thank you for your enquiry. I have obtained the following information to help answer your questions: 1) Each kit can test 50 samples. i.e. 100/2, minus the wells for background and the Hela nuclear extract used for a standard 2) If you use a...

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