Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Nuclear Extracts, Purified protein, Tissue Lysate
Product nameHistone Acetyltransferase Activity Assay Kit (Colorimetric)
See all Histone acetyltransferase kits
Sample typeNuclear Extracts, Purified protein, Tissue Lysate
Assay typeEnzyme activity
Species reactivityReacts with: Mammals, Other species
Abcam's Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
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HAT assay protocol summary:
- add samples to wells
- add HAT assay mix
- incubate for 1-4 hrs
- analyze with microplate reader
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Storage instructionsStore at -80°C. Please refer to protocols.
Components Identifier 100 tests 2X HAT Assay Buffer Amber 1 x 7.5ml HAT Reconstitution Buffer Clear 1 x 1.8ml HAT Substrate I Blue 1 vial HAT Substrate II Red 1 vial NADH Generating Enzyme Green 1 x 800µl Nuclear Extract (4 mg/ml ) Violet 1 x 50µl
RelevanceHistone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Cellular localizationCytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
- Histone acetyltransferase 1
HAT activity in NIH3T3 cells, which were transfected with the desired plasmids by using Lipofectamine 2000. After 24 hours from transfection, the nuclear protein was extracted and the Histone acetyltransferase activity was measured by using ab65352.
Histone acetylation activity assay for L.donovani cells over-expressing HAT1, HAT2, HAT3 and HAT4. The activities were compared to wild-type (WT) cells and pLPneo2 (vector) only transfected cells. The relative activity was determined using Histone Acetyltransferase acitvity assay kit (ab65352).
Analysis of Histone Acetyltransferase Activity in HeLa Nuclear Extract using ab65352. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a micro plate reader at 440 nm according to the kit instructions.
ab65352 has been referenced in 17 publications.
- Pao PC et al. HDAC1 modulates OGG1-initiated oxidative DNA damage repair in the aging brain and Alzheimer's disease. Nat Commun 11:2484 (2020). PubMed: 32424276
- Smith ER et al. TGF-ß1 modifies histone acetylation and acetyl-coenzyme A metabolism in renal myofibroblasts. Am J Physiol Renal Physiol N/A:N/A (2019). PubMed: 30623724
- Alrdahe S et al. Dysregulation of macrophage development and phenotype in diabetic human macrophages can be rescued by Hoxa3 protein transduction. PLoS One 14:e0223980 (2019). PubMed: 31626638
- Verhoeven RJA et al. Epstein-Barr Virus BART Long Non-coding RNAs Function as Epigenetic Modulators in Nasopharyngeal Carcinoma. Front Oncol 9:1120 (2019). PubMed: 31696060
- Jha PK et al. HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani. PLoS One 12:e0177372 (2017). Leishmania . PubMed: 28486547