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I have some questions about this product. 1. How many samples can I run for each kit? (if duplicate samples) 2. Our lab has only spectrometer at 405nm. Is this OK to read the result? 3. Can I use whole cell abstract instead of nuclear extract?
Asked on Feb 15 2010
Thank you for your enquiry. I have obtained the following information to help answer your questions: 1) Each kit can test 50 samples. i.e. 100/2, minus the wells for background and the Hela nuclear extract used for a standard 2) If you use a spectrophotometer at 405 nm, whether you will be able to accurately read the results will depend on the bandwidth of the filter your reader has. If it has a 30 nm bandwidth that would cover 440 nm; if the band width is very small, may not be optimal. I can recommend to check this. 3) I can suggest that whole cell extracts will probably give more background and the results may not be as clear. As HAT is a nuclear protein, we would recommend to try nuclear extract.
Answered on Feb 15 2010