Overview

  • Product name

    Histone Acetyltransferase Activity Assay Kit (Fluorometric)
    See all Histone acetyltransferase kits
  • Detection method

    Fluorescent
  • Sample type

    Nuclear Extracts, Purified protein
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709) utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from AcetylCoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.

  • Notes

    Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases.

  • Platform

    Microplate reader

Properties

Images

  • Typical CoA Standard calibration curve.

  • HAT Activity in HeLa Nuclear Extract.

  • Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF.

Protocols

References

ab204709 has not yet been referenced specifically in any publications.

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