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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation HAT
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Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)

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  • SDS
  • Protocol Booklet
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Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
  • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
  • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Nuclear Extracts, Purified protein

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Overview

  • Product name

    Histone Acetyltransferase Activity Assay Kit (Fluorometric)
    See all Histone acetyltransferase kits
  • Detection method

    Fluorescent
  • Sample type

    Nuclear Extracts, Purified protein
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709) utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from AcetylCoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K334 HAT Activity Fluorometric Assay Kit. K334-100 is the same size as the 100 test size of ab204709.

    Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 100 tests
    Acetyl CoA (Lyophilized) 1 vial
    CoA Standard (Lyophilized) 1 vial
    Developer 1 x 100µl
    H3 Peptide (Lyophilized) 1 vial
    HAT Assay Buffer 1 x 25ml
    PicoProbe 1 x 200µl
    Positive Control (HeLa Nuclear Extract) 1 x 40µl
    Substrate Mix (Lyophilized) 1 vial
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Amino Acid Assays
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • Histone acetylation and deacetylation
  • Relevance

    Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
  • Cellular localization

    Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
  • Alternative names

    • HAT
    • HAT1
    • Histone acetyltransferase 1
    • Histone acetyltransferase type B catalytic subunit
    • KAT1
    see all

Images

  • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
    Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)

    Typical CoA Standard calibration curve.

  • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
    Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)

    HAT Activity in HeLa Nuclear Extract.

  • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
    Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)

    Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab204709? Please let us know so that we can cite the reference in this datasheet.

ab204709 has not yet been referenced specifically in any publications.

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