Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Nuclear Extracts, Purified protein
Product nameHistone Acetyltransferase Activity Assay Kit (Fluorometric)
See all Histone acetyltransferase kits
Sample typeNuclear Extracts, Purified protein
Assay typeEnzyme activity
Species reactivityReacts with: Mammals, Other species
Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709) utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from AcetylCoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K334 HAT Activity Fluorometric Assay Kit. K334-100 is the same size as the 100 test size of ab204709.
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases.
Storage instructionsStore at -80°C. Please refer to protocols.
Components 100 tests Acetyl CoA (Lyophilized) 1 vial CoA Standard (Lyophilized) 1 vial Developer 1 x 100µl H3 Peptide (Lyophilized) 1 vial HAT Assay Buffer 1 x 25ml PicoProbe 1 x 200µl Positive Control (HeLa Nuclear Extract) 1 x 40µl Substrate Mix (Lyophilized) 1 vial
RelevanceHistone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Cellular localizationCytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
- Histone acetyltransferase 1
ab204709 has not yet been referenced specifically in any publications.