Overview

  • Product name

    Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric, Histone H4)
    See all Histone acetyltransferase kits
  • Detection method

    Fluorescent
  • Sample type

    Cell culture supernatant, Cell culture extracts, Tissue, Cell Lysate, Cell culture media
  • Product overview

    Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric, Histone H4) (ab239713) utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and Probe to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm.


    The assay can detect HAT activity equivalent to as low as 1.5 U of p300 in a variety of samples.


     


     


     

  • Platform

    Microplate reader

Properties

Images

Protocols

References

ab239713 has not yet been referenced specifically in any publications.

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