Product nameHistone Acetyltransferase Inhibitor Screening Assay Kit
See all Histone acetyltransferase kits
Intra-assay Sample n Mean SD CV% HAT 16 3.2% Inter-assay Sample n Mean SD CV% HAT 16 4.4%
Assay typeEnzyme activity
Histone Acetyltransferase Inhibitor Screening Kit (ab133099) provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors.
The procedure is based on the ability of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) to react with free thiol grious present after the acetylation reaction. The reaction of the CPM reacts these free thiol groups forms a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 nm and 450-470 nm, respectively.
Storage instructionsPlease refer to protocols.
Components 96 tests 96-Well Plate (white) 1 unit 96-Well Plate Cover 1 unit HAT Acetyl CoA 1 vial HAT Assay Buffer (5X) 1 vial HAT Developer 1 vial HAT Peptide 1 vial HAT Stop Reagent 1 vial Histone Acetyltransferase (pCAF) 1 vial
RelevanceHistone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Cellular localizationCytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
- Histone acetyltransferase 1
ab133099 has not yet been referenced specifically in any publications.