Overview

  • Product name

    Histone Acetyltransferase Inhibitor Screening Assay Kit
    See all Histone acetyltransferase kits
  • Detection method

    Fluorescent
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HAT 16 3.2%
    Inter-assay
    Sample n Mean SD CV%
    HAT 16 4.4%
  • Assay type

    Enzyme activity
  • Product overview

     Histone Acetyltransferase Inhibitor Screening Kit (ab133099) provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors.

    The procedure is based on the ability of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) to react with free thiol grious present after the acetylation reaction. The reaction of the CPM reacts these free thiol groups forms a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 nm and 450-470 nm, respectively.

  • Platform

    Microplate reader

Properties

Images

  • An example of a known pCAF HAT inhibitor, garcinol, interfering with the assay.

Protocols

References

ab133099 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

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