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    histone-core-antibody-ab7832.pdf

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Epigenetics and Nuclear Signaling Histones Core
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Anti-Histone Core antibody (ab7832)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (2)

Product price, shipping and contact information

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Key features and details

  • Sheep polyclonal to Histone Core
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

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Secondary
Product image
Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

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Overview

  • Product name

    Anti-Histone Core antibody
  • Description

    Sheep polyclonal to Histone Core
  • Host species

    Sheep
  • Specificity

    The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.
  • Species reactivity

    Reacts with: Mouse, Cow, Human
  • Immunogen

    Human histone.

  • Positive control

    • Tonsil, colon carcinoma and any other human tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Core

Associated products

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)

Target

  • Relevance

    In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.
  • Cellular localization

    Nuclear

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab7832? Please let us know so that we can cite the reference in this datasheet.

ab7832 has been referenced in 2 publications.

  • Zheng S  et al. MIR31HG promotes cell proliferation and invasion by activating the Wnt/ß-catenin signaling pathway in non-small cell lung cancer. Oncol Lett 17:221-229 (2019). PubMed: 30655759
  • Li Y  et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . PubMed: 18478156

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Histone Core antibody

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
10 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Jul 13 2007

Question

I was wondering if ab7832 recognizes histone H2A. The data sheet does not list it as being recognized by the anitbody.

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Abcam community

Verified customer

Asked on Nov 18 2002

Answer

The antibody (ab7832 -- Sheep polyclonal to Histone Core)binds to H1, H2B, H3 and H4; however, there is no evidence to suggest that the antibody can bind to H2A.

Read More

Abcam Scientific Support

Answered on Dec 11 2002

Question

I am looking for antibody to recognize the core histones in Drosophila melanogaster. Has this antibody been tested in this species?

Read More

Abcam community

Verified customer

Asked on Jul 09 2002

Answer

This antibody has not been tested specifically in Drosophila, but it works in both animal and plant tissue, so I would expect it to work in Drosophila. Core histones are highly conserved.

Read More

Abcam Scientific Support

Answered on Jul 11 2002

Question

I'm interested in finding an antibody that will recognize the HFD of S. cerevisiae H4 expressed in E. coli. Will this antibody do this?

Read More

Abcam community

Verified customer

Asked on May 03 2002

Answer

This antibody has not been tested in budding yeast, however as histones are highly conserved across species, it is quite likely that the antibody will react with yeast histones. As the antibody is raised against a mixture of histones, I think it likely that it will recognise the histone fold domain.

Read More

Abcam Scientific Support

Answered on May 06 2002

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