Anti-Histone Core antibody (ab7832)
Key features and details
- Sheep polyclonal to Histone Core
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone Core antibody -
Description
Sheep polyclonal to Histone Core -
Host species
Sheep -
Specificity
The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A. -
Species reactivity
Reacts with: Mouse, Cow, Human -
Immunogen
Human histone.
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Positive control
- Tonsil, colon carcinoma and any other human tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Target
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Relevance
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species. -
Cellular localization
Nuclear
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab7832 has been referenced in 2 publications.
- Zheng S et al. MIR31HG promotes cell proliferation and invasion by activating the Wnt/ß-catenin signaling pathway in non-small cell lung cancer. Oncol Lett 17:221-229 (2019). PubMed: 30655759
- Li Y et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . PubMed: 18478156