Anti-Histone Core antibody (ab7832)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Histone Core antibody -
Description
Sheep polyclonal to Histone Core -
Host species
Sheep -
Specificity
The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A. -
Tested applications
Suitable for: IP, WB, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Mouse, Cow, Human -
Immunogen
Human histone.
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Positive control
- Tonsil, colon carcinoma and any other human tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab7832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | ||
| WB | ||
| ICC/IF | ||
| ELISA |
Application notes
ELISA: 1/1000.
ICC/IF: Use at an assay dependent dilution (PMID 18478156).
IP: Use at an assay dependent dilution.
WB: 1/200.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (PMID 18478156).
IP: Use at an assay dependent dilution.
WB: 1/200.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species. -
Cellular localization
Nuclear
Images
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Western blot - Anti-Histone Core antibody (ab7832)This image is courtesy of an anonymous Abreviewab7832 at 1/200 staining human HL60 whole cell lysate by Western blot (10ug/lane). The antibody was incubated for 16 hours. Abcam antibody ab6747 (rabbit polyclonal to sheep IgG (HRP)) was used as the secondary.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zheng S et al. MIR31HG promotes cell proliferation and invasion by activating the Wnt/ß-catenin signaling pathway in non-small cell lung cancer. Oncol Lett 17:221-229 (2019). Read more (PubMed: 30655759) »
- Li Y et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . Read more (PubMed: 18478156) »
