Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
10 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA TBS
Secondary antibody
Name
Dilution
1/2000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 14, 17 kDa Non-specific: 28, 29, ~50 kDa
Additional data
Additional Notes
Cell lysed in RIPA buffer supplemented with Complete Protease Inhibitor Cocktail Tablets (Roche #11 836 153 001) & phosphatase inhibitors (Sigma #P2850 & P5726)
Nitrocellulose membrane
Good signal. Couldn't detect H1 though.
Nitrocellulose membrane
Good signal. Couldn't detect H1 though.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jul 13 2007