For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064) detects HDAC activity in lysates. Primarily, the Histone Deacetylase (HDAC) Activity Assay Kit is designed for the rapid and sensitive evaluation of HDAC inhibitors using crude HDAC fraction. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC activity with the Histone Deacetylase (HDAC) Activity Assay Kit if the appropriate dose of HDAC specific inhibitor e.g. Trichostatin A is used.
Abcam’s Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) has been shown to detect the activity of HDAC family, at least class I HDACs in Human or animal cell lysates or in column fractions. The assay shows good linearity of sample response. The assay may be used to follow the purification of HDACs or may be used to detect the presence of HDACs in cell lysates
Applications for this kit include:
1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8.
2. Screening inhibitors or activators of HDACs.
3. Detecting the effects of pharmacological agents on HDACs.
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
|Crude HDAC||1 x 500µl|
|Developer||1 x 500µl|
|Fluoro-Deacetylated Peptide (0.2 mM)||1 x 100µl|
|Fluoro-Substrate Peptide (0.2 mM)||1 x 500µl|
|HDAC Assay Buffer||2 x 1ml|
|HDAC Stop Solution||2 x 1ml|
|Trichostatin A (10µM)||1 x 500µl|
8 uL (corresponding to 6.7e6 cells) of nuclear extracts (ab113474) from HL60 cells were assessed kinetically (+/- SD).
Different volumes of positive control, with inhibitor (TA), kinetically read (+/- SD).
Measurement of HeLa cell endogenous HDAC1 in an immunoprecipitated sample with anti-HDAC1 antibody and following the Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064) protocol.
Measurement of HeLa cell endogenous HDAC2 in an immunoprecipitated sample with anti-HDAC2 antibody following the Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064) protocol.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"