Overview

  • Product name
    Histone Demethylase (H3K4) Activity Quantification Assay Kit
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Enzyme activity
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: Mammals, Other species
  • Product overview

    Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.


    Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.


     

  • Platform
    Microplate reader

Properties

Images

  • Nuclear extracts were prepared from Hela cells using the ab113475 and H3K4 specific histone demethyase activity was measured.

Protocols

References

ab113455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your interest in ab113455 & ab113464.

You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimethylated histone H3-K4 substrate while the substrate used in ab113464 is a trimethylated histone H3-K4 substrate.

Due to these different substrates, ab113455 will detect LSD1 type demethylase activity which can remove mono and dimethyl groups while ab113464 will detect JARID type demethylase activity which removes trimethyl groups from H3 K4.

So it seems ab113464 would be the proper choice for your interest in studying H3K4me3 demethylation activity.

As far as we know neither of these kits have been used in published works.

I hope this is helpful. Please contact us again if you have any further questions.

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