Histone Extraction Kit (ab113476)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
-
Product name
Histone Extraction Kit
See all Histone Extraction kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay time
1h 00m -
Product overview
Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.
The amount of starting material can be as low as 105 cells or 1 mg of tissue. For best results, the cell number should be >106 cells or the tissue amount should be >10 mg.
A total of 100 standard extractions (using 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.
-
Notes
Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.
Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 10X Pre-Lysis Buffer 1 x 10ml Balance Buffer 1 x 8ml DTT Solution 1 x 20µl Lysis Buffer 1 x 20ml -
Research areas
Images
-
Functional Studies - Histone Extraction Kit (ab113476)Nowacka-Woszuk, Joanna et al., PloS one?vol. 13,2 e0193464., Fig 4, doi:10.1371/journal.pone.0193464
The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using ab115124
Asterisk indicate a significant difference between the respective C and R groups: ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group). -
SDS-PAGE Analysis of samples prepared using Histone extraction KitSDS-PAGE Analysis of samples prepared using Histone extraction Kit. -
Functional Studies - Histone Extraction KitHistones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.
Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE -
Functional Studies - Histone Extraction Kit (ab113476)Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.
Datasheets and documents
-
SDS download
-
Datasheet download
References (64)
ab113476 has been referenced in 64 publications.
- Ehteda A et al. Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG. Cell Rep 35:108994 (2021). PubMed: 33852836
- Yang H et al. Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring. Int J Mol Sci 22:N/A (2021). PubMed: 34445278
- Antonelli R et al. CN133, a Novel Brain-Penetrating Histone Deacetylase Inhibitor, Hampers Tumor Growth in Patient-Derived Pediatric Posterior Fossa Ependymoma Models. Cancers (Basel) 12:N/A (2020). PubMed: 32708733
- An S et al. Histone tail analysis reveals H3K36me2 and H4K16ac as epigenetic signatures of diffuse intrinsic pontine glioma. J Exp Clin Cancer Res 39:261 (2020). PubMed: 33239043
- Metz PJ et al. Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling. Cell Rep 30:1935-1950.e8 (2020). PubMed: 32049022