2.5 g leaf material was frozen and ground in liquid N2 and used for the extraction with 8 ml 1x Pre-Lysis Buffer. Two filtration steps through 100 μm and 30 μm nylon meshes and additional Pre-Lysis Buffer wash steps were performed in order to remove residual plant material.
The histone extract was precipitated with 4% perchloric acid o/n.
The Histone Extraction Kit is easy to use and generally works with A. thaliana leaves. However, the resulting sample contained some unexpected proteins, e.g. chloroplastic proteins, in addition to histones.
Submitted Sep 28 2017