Overview

  • Product name

    Anti-Histone H1 (acetyl K52) antibody
  • Description

    Rabbit polyclonal to Histone H1 (acetyl K52)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Histone H1 aa 30-60 (acetyl K55) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.

  • Positive control

    • Human kidney and rat spleen tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 0.01% BSA

    Aqueous buffered solution
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Relevance

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • H1 antibody
    • H1 histone family member 5 antibody
    • H1 histone family, member 0 antibody
    • H1.0, H1(0), H1-0 antibody
    • H10 antibody
    • H15 antibody
    • H1F5 antibody
    • H1FV antibody
    • HIST1H1B antibody
    • histone 1 antibody
    • Histone H1 0 antibody
    • Histone H1 antibody
    • Histone H1' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • histone H1a antibody
    see all

References

This product has been referenced in:

  • Xiang Y  et al. Acetylpuerarin inhibits oxygen-glucose deprivation-induced neuroinflammation of rat primary astrocytes via the suppression of HIF-1 signaling. Exp Ther Med 16:2689-2695 (2018). Read more (PubMed: 30210610) »
  • Yang B  et al. Caffeoylxanthiazonoside exerts cardioprotective effects during chronic heart failure via inhibition of inflammatory responses in cardiac cells. Exp Ther Med 14:4224-4230 (2017). Read more (PubMed: 29104638) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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