Overview

  • Product name
  • Description
    Rabbit polyclonal to Histone H1
  • Host species
    Rabbit
  • Specificity
    Detects total Histone H1 levels.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (E-K-TP-P-V).

  • Positive control
    • IHC-P: Human normal colon FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab61177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

Images

  • All lanes : Anti-Histone H1 antibody (ab61177) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with synthesized peptide

    Predicted band size: 36 kDa

  • IHC image of ab61177 staining Histone H1 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab61177 at 1/500 - 1/1000 dilution staining Histone H1 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.
  • Paraffin-embedded human colon carcinoma tissue stained for Histone H1 with ab61177 at 1/50 dilution in immunohistochemical analysis.

    The sample in the right-hand panel is incubated with the immunizing peptide.

References

This product has been referenced in:
  • Shinjo T  et al. Propofol induces nuclear localization of Nrf2 under conditions of oxidative stress in cardiac H9c2 cells. PLoS One 13:e0196191 (2018). Read more (PubMed: 29689082) »
  • El-Daher MT  et al. Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure. Cell Discov 4:61 (2018). Read more (PubMed: 30455981) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Human Cell lysate - nuclear (huh7 liver)
Specification
huh7 liver
Treatment
infected with flavivirus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2014

Answer

Thank you for your enquiry.

Based on the immunogen sequence, ab61177 should recognize
Histone H1.5 (http://www.uniprot.org/uniprot/P16401),
Histone H1.3 (http://www.uniprot.org/uniprot/P16402), and
Histone H1.4 (http://www.uniprot.org/uniprot/P10412).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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