Anti-Histone H1 antibody [AE-4] (ab71594)

IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue^* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

ab71594 staining Histone H1 in HeLa cells by Immunocytochemistry/Electron Microscopy. Cells were frozen under high pressure. Samples were incubated with ab71594 at 5µg/ml in 1% serum, 1% BSA, PBS. The secondary used was a donkey anti-mouse conjugated to 10nM gold, 1/30 dilution.