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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Non Homol. End Joining
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Anti-Histone H1 antibody [AE-4] (ab71594)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (17)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)

    Key features and details

    • Mouse monoclonal [AE-4] to Histone H1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-Histone H1 antibody [AE-4]
      See all Histone H1 primary antibodies
    • Description

      Mouse monoclonal [AE-4] to Histone H1
    • Host species

      Mouse
    • Specificity

      Histone H1 itself is a highly conserved protein and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Nuclei from myeloid leukamia biopsy cells.

    • Positive control

      • IHC-P: Human normal colon FFPE tissue sections.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      AE-4
    • Isotype

      IgG2a
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Non Homol. End Joining
      • Epigenetics and Nuclear Signaling
      • Histones
      • H1
      • Unmodified

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human Histone H1 protein (ab198676)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab71594 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration.

    Target

    • Function

      May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
    • Tissue specificity

      Oocyte-specific.
    • Sequence similarities

      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Cellular localization

      Cytoplasm. Nucleus. Chromosome.
    • Target information above from: UniProt accession Q8IZA3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 132243 Human
      • Entrez Gene: 3005 Human
      • Entrez Gene: 3006 Human
      • Entrez Gene: 3007 Human
      • Entrez Gene: 3008 Human
      • Entrez Gene: 3009 Human
      • Entrez Gene: 3010 Human
      • Entrez Gene: 3024 Human
      • Entrez Gene: 341567 Human
      • Entrez Gene: 8971 Human
      • Omim: 142210 Human
      • Omim: 142220 Human
      • Omim: 142708 Human
      • Omim: 142709 Human
      • Omim: 142710 Human
      • Omim: 142711 Human
      • Omim: 602785 Human
      • SwissProt: P07305 Human
      • SwissProt: P10412 Human
      • SwissProt: P16401 Human
      • SwissProt: P16402 Human
      • SwissProt: P16403 Human
      • SwissProt: P22492 Human
      • SwissProt: Q02539 Human
      • SwissProt: Q147U8 Human
      • SwissProt: Q2M2I2 Human
      • SwissProt: Q3MJ34 Human
      • SwissProt: Q3MJ42 Human
      • SwissProt: Q4VB25 Human
      • SwissProt: Q6FG88 Human
      • SwissProt: Q6ISI1 Human
      • SwissProt: Q7Z694 Human
      • SwissProt: Q86WT7 Human
      • SwissProt: Q8IZA3 Human
      • SwissProt: Q8N6R3 Human
      • SwissProt: Q92522 Human
      • Unigene: 97358 Human
      see all
    • Alternative names

      • dJ221C16.2 antibody
      • dJ221C16.5 antibody
      • DmeH1 antibody
      • H1 0 histone antibody
      • H1 antibody
      • H1 histone family member 0 antibody
      • H1 histone family member 1 antibody
      • H1 histone family member 3 antibody
      • H1 histone family member 5 antibody
      • H1 histone family member N testis specific antibody
      • H1 histone family member O oocyte specific antibody
      • H1 histone family member X antibody
      • H1(0) antibody
      • H1.1 antibody
      • H1.2 antibody
      • H1.3 antibody
      • H1.4 antibody
      • H1.5 antibody
      • H10 antibody
      • H1A antibody
      • H1f0 antibody
      • H1F0 protein antibody
      • H1F1 antibody
      • H1F2 antibody
      • H1F3 antibody
      • H1F4 antibody
      • H1F5 antibody
      • H1FNT antibody
      • H1FOO antibody
      • H1FOO protein antibody
      • H1FOO_HUMAN antibody
      • H1FT antibody
      • H1FV antibody
      • H1FX antibody
      • H1oo antibody
      • H1t antibody
      • H1T2 antibody
      • H1X antibody
      • HANP1 antibody
      • His1 antibody
      • HisC antibody
      • HIST1 antibody
      • HIST1H1A antibody
      • HIST1H1B antibody
      • HIST1H1C antibody
      • HIST1H1D antibody
      • HIST1H1E antibody
      • HIST1H1T antibody
      • Histone 1 H1a antibody
      • Histone 1 H1b antibody
      • Histone 1 H1c antibody
      • Histone 1 H1d antibody
      • Histone 1 H1e antibody
      • Histone 1 H1t antibody
      • Histone antibody
      • Histone H1' antibody
      • Histone H1.0 antibody
      • Histone H1.1 antibody
      • Histone H1.2 antibody
      • Histone H1.3 antibody
      • Histone H1.4 antibody
      • Histone H1.5 antibody
      • Histone H1oo antibody
      • Histone H1x antibody
      • MGC116819 antibody
      • MGC126630 antibody
      • MGC126632 antibody
      • MGC126642 antibody
      • MGC138176 antibody
      • MGC138345 antibody
      • MGC15959 antibody
      • MGC3992 antibody
      • MGC50807 antibody
      • MGC5241 antibody
      • MGC8350 antibody
      • Oocyte specific histone H1 antibody
      • Oocyte specific linker histone H1 antibody
      • Oocyte-specific histone H1 antibody
      • Oocyte-specific linker histone H1 antibody
      • osH1 antibody
      • Testicular H1 histone antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)

      IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (17)

    Publishing research using ab71594? Please let us know so that we can cite the reference in this datasheet.

    ab71594 has been referenced in 17 publications.

    • Senigagliesi B  et al. The High Mobility Group A1 (HMGA1) Chromatin Architectural Factor Modulates Nuclear Stiffness in Breast Cancer Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31167352
    • Wu F  et al. miR-501-3p promotes colorectal cancer progression via activation of Wnt/ß-catenin signaling. Int J Oncol 55:671-683 (2019). PubMed: 31364752
    • Perri AM  et al. Histone proteomics reveals novel post-translational modifications in breast cancer. Aging (Albany NY) 11:11722-11755 (2019). PubMed: 31816600
    • Dicker AJ  et al. Neutrophil extracellular traps are associated with disease severity and microbiota diversity in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol 141:117-127 (2018). PubMed: 28506850
    • Fišerová J  et al. Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 130:2066-2077 (2017). PubMed: 28476938
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Histone H1 antibody [AE-4]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Herr Dr. Paul Vesely

    Verified customer

    Submitted Feb 23 2016

    Question

    Would you please help to verify the following questions regarding your
    anti-histone H1 anti bodies:
    Anti-Histone H1 antibody (ab87556): Detects a ˜32KD band;
    Anti-Histone H1 antibody (ab83058) : Detects a ˜24KD band;
    Anti-Histone H1 antibody [1415-1] - Carboxyterminal end (ab62884) :
    Detects a ˜17KD band;
    Anti-Histone H1 antibody [AE-4] (ab71594) : no WB image;
    Anti-Histone H1 antibody [EPR6537] (ab125027): WB ˜32KD
    1). Do they represent different modifications of H1 or different isofroms
    of H1, since the band sizes recognized are so diffenrent? (But MW of H1
    isoforms are around 22KD).
    2). Will above antibodies also cross-react with other histones like H3 or H4?
    Anti-Histone H1 antibody [0.T.60] (ab4269): Detects isoforms 0-5,
    ˜25-32KD; Why the sizes are different from the oredicted sizes, or due to
    modification or something else?
    In the end, we need an antibody to detect Histone H1 in human cell lines
    such as HEK293 and SH-SY5Y, which one do you recommend?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 05 2012

    Answer

    Thank you for contacting us.

    ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

    ab83058; the sequence used is from P07305 http://www.uniprot.org/uniprot/P07305. The molecular weight is ˜ 21kDa.

    ab71594 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is ˜ 35 kDa.

    ab125027 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is ˜ 35 kDa.

    These antibodies are specific to human H1 protein only not the modifications. You can purchase any of these to detect H1 human protein.

    Read More

    Abcam Scientific Support

    Answered on Mar 05 2012

    Immunocytochemistry abreview for Anti-Histone H1 antibody [AE-4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa cells)
    Specification
    HeLa cells
    Fixative
    high-pressure freezing
    Permeabilization
    No
    Read More

    Dr. Margarita Sobol

    Verified customer

    Submitted Jan 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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