Anti-Histone H1 antibody [AE-4] (ab71594)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)](https://www.abcam.com/ps/products/71/ab71594/Images/ab71594-229360-anti-histone-h1-antibody-ae-4-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)")
Key features and details
- Mouse monoclonal [AE-4] to Histone H1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Histone H1 antibody [AE-4]
See all Histone H1 primary antibodies -
Description
Mouse monoclonal [AE-4] to Histone H1 -
Host species
Mouse -
Specificity
Histone H1 itself is a highly conserved protein and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Nuclei from myeloid leukamia biopsy cells.
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Positive control
- IHC-P: Human normal colon FFPE tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AE-4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
Target
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Function
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling. -
Tissue specificity
Oocyte-specific. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Cytoplasm. Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 132243 Human
- Entrez Gene: 3005 Human
- Entrez Gene: 3006 Human
- Entrez Gene: 3007 Human
- Entrez Gene: 3008 Human
- Entrez Gene: 3009 Human
- Entrez Gene: 3010 Human
- Entrez Gene: 3024 Human
see all -
Alternative names
- dJ221C16.2 antibody
- dJ221C16.5 antibody
- DmeH1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)
IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Protocols
References (17)
ab71594 has been referenced in 17 publications.
- Senigagliesi B et al. The High Mobility Group A1 (HMGA1) Chromatin Architectural Factor Modulates Nuclear Stiffness in Breast Cancer Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31167352
- Wu F et al. miR-501-3p promotes colorectal cancer progression via activation of Wnt/ß-catenin signaling. Int J Oncol 55:671-683 (2019). PubMed: 31364752
- Perri AM et al. Histone proteomics reveals novel post-translational modifications in breast cancer. Aging (Albany NY) 11:11722-11755 (2019). PubMed: 31816600
- Dicker AJ et al. Neutrophil extracellular traps are associated with disease severity and microbiota diversity in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol 141:117-127 (2018). PubMed: 28506850
- Fišerová J et al. Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 130:2066-2077 (2017). PubMed: 28476938
