Anti-Histone H1 antibody [AE-4] (ab71594)
- Datasheet
- Specific References (11)
- Protocols
Overview
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Product nameAnti-Histone H1 antibody [AE-4]
See all Histone H1 primary antibodies -
DescriptionMouse monoclonal [AE-4] to Histone H1
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Host speciesMouse
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SpecificityHistone H1 itself is a highly conserved protein and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
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Tested applicationsSuitable for: IHC-Fr, IHC-P, ELISA, WB, ICC, ICC/IF, Electron Microscopymore details
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Species reactivityReacts with: Human
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Immunogen
Nuclei from myeloid leukamia biopsy cells.
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Positive control
- IHC-P: Human normal colon FFPE tissue sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberAE-4
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab71594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | 1/100. | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 21063722 |
Target
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FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
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Tissue specificityOocyte-specific.
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Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localizationCytoplasm. Nucleus. Chromosome.
- Information by UniProt
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Database links
- Entrez Gene: 132243 Human
- Entrez Gene: 3005 Human
- Entrez Gene: 3006 Human
- Entrez Gene: 3007 Human
- Entrez Gene: 3008 Human
- Entrez Gene: 3009 Human
- Entrez Gene: 3010 Human
- Entrez Gene: 3024 Human
see all -
Alternative names
- dJ221C16.2 antibody
- dJ221C16.5 antibody
- DmeH1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)](https://www.abcam.com/ps/products/71/ab71594/Images/ab71594-229360-71594gr12779431in1000ed.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [AE-4] (ab71594)IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Electron Microscopy - Anti-Histone H1 antibody [AE-4] (ab71594)](https://www.abcam.com/ps/products/71/ab71594/Images/Histone-H1-Primary-antibodies-ab71594-4.jpg)
Electron Microscopy - Anti-Histone H1 antibody [AE-4] (ab71594)Image courtesy of Margarita Sobol by Abreview.ab71594 staining Histone H1 in HeLa cells by Immunocytochemistry/Electron Microscopy. Cells were frozen under high pressure. Samples were incubated with ab71594 at 5µg/ml in 1% serum, 1% BSA, PBS. The secondary used was a donkey anti-mouse conjugated to 10nM gold, 1/30 dilution.
Protocols
References
This product has been referenced in:
- Dicker AJ et al. Neutrophil extracellular traps are associated with disease severity and microbiota diversity in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol 141:117-127 (2018). Read more (PubMed: 28506850) »
- Pellegrini L et al. HMGB1 targeting by ethyl pyruvate suppresses malignant phenotype of human mesothelioma. Oncotarget 8:22649-22661 (2017). Read more (PubMed: 28186988) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"