Anti-Histone H1.0 antibody (ab154111)
Key features and details
- Rabbit polyclonal to Histone H1.0
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Histone H1.0 antibody
See all Histone H1.0 primary antibodies -
Description
Rabbit polyclonal to Histone H1.0 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Histone H1.0 aa 1-194. The exact sequence is proprietary.
Database link: P07305 -
Positive control
- HepG2 whole cell lysate; HepG2 nuclear lysate; U20S cells; SkHep1 xenograft; C2C12 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsPhosphorylated on Ser-17 in RNA edited version. -
Cellular localization
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Omim: 142708 Human
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- Unigene: 745024 Human
- Unigene: 24350 Mouse
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Alternative names
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
Images
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All lanes : Anti-Histone H1.0 antibody (ab154111) at 1/5000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa -
Immunofluorescent analysis of paraformaldehyde fixed U20S cells labeling Histone H1 with ab154111 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2000.
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Immunohistochemical analysis of paraffin embedded SkHep1 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.
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Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154111 has not yet been referenced specifically in any publications.