Overview

  • Product name

    Anti-Histone H1.0 antibody [EPR6536]
    See all Histone H1.0 primary antibodies
  • Description

    Rabbit monoclonal [EPR6536] to Histone H1.0
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human Histone H1.0 (UniProt: P07305).

  • Positive control

    • Human fetal kidney and BxPC3 cell lysates; Human colon tissue; BxPC3 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
    • Sequence similarities

      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-17 in RNA edited version.
    • Cellular localization

      Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • H1 histone family member 0 antibody
      • H1(0) antibody
      • H10 antibody
      • H10_HUMAN antibody
      • h1f0 antibody
      • H1FV antibody
      • Histone H1'' antibody
      • Histone H1(0) antibody
      • Histone H1.0 antibody
      • Histone H10 antibody
      • Histone H5 antibody
      • MGC5241 antibody
      • N-terminally processed antibody
      see all

    Images

    • All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : BcPC3 cel lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 21 kDa

    • Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.

    References

    This product has been referenced in:

    • Fukui K  et al. Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells. J Clin Biochem Nutr 64:36-44 (2019). Read more (PubMed: 30705510) »
    • Tanaka S  et al. Trim33 mediates the proinflammatory function of Th17 cells. J Exp Med 215:1853-1868 (2018). Read more (PubMed: 29930104) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    mouse embryonic stem cells (J1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jan 04 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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