Recombinant Anti-Histone H1.0 antibody [EPR6536] (ab134914)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6536] to Histone H1.0
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Histone H1.0 antibody [EPR6536]
See all Histone H1.0 primary antibodies -
Description
Rabbit monoclonal [EPR6536] to Histone H1.0 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 and HeLa cell lysates; Human fetal kidney and BxPC3 cell lysates. IHC-P: Human colon tissue. ICC/IF: BxPC3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6536 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsPhosphorylated on Ser-17 in RNA edited version. -
Cellular localization
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Entrez Gene: 24437 Rat
- Omim: 142708 Human
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- SwissProt: P43278 Rat
- Unigene: 745024 Human
see all -
Alternative names
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
Images
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All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : H1F0 knockout A431 cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 A431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : BcPC3 cel lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 21 kDa -
Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab134914 has been referenced in 6 publications.
- Morales Torres C et al. Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. Nat Commun 11:1792 (2020). PubMed: 32286289
- Cangkrama M et al. A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming. EMBO Mol Med 12:e11466 (2020). PubMed: 32150356
- Fukui K et al. Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells. J Clin Biochem Nutr 64:36-44 (2019). PubMed: 30705510
- Knopf J et al. Aggregated NETs Sequester and Detoxify Extracellular Histones. Front Immunol 10:2176 (2019). PubMed: 31572386
- Tanaka S et al. Trim33 mediates the proinflammatory function of Th17 cells. J Exp Med 215:1853-1868 (2018). PubMed: 29930104
- He X et al. Activation of nuclear factor kappa B in the hepatic stellate cells of mice with schistosomiasis japonica. PLoS One 9:e104323 (2014). WB ; Mouse . PubMed: 25116007