Product nameAnti-Histone H1.0 antibody [EPR6536]
See all Histone H1.0 primary antibodies
DescriptionRabbit monoclonal [EPR6536] to Histone H1.0
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the N terminus of Human Histone H1.0 (UniProt: P07305).
- Human fetal kidney and BxPC3 cell lysates; Human colon tissue; BxPC3 cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
modificationsPhosphorylated on Ser-17 in RNA edited version.
Cellular localizationNucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
- Information by UniProt
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : BcPC3 cel lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 21 kDa
Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.
This product has been referenced in:
- Fukui K et al. Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells. J Clin Biochem Nutr 64:36-44 (2019). Read more (PubMed: 30705510) »
- Tanaka S et al. Trim33 mediates the proinflammatory function of Th17 cells. J Exp Med 215:1853-1868 (2018). Read more (PubMed: 29930104) »