Recombinant
RabMAb

Recombinant Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)

Overview

  • Product name

    Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free
    See all Histone H1.0 primary antibodies
  • Description

    Rabbit monoclonal [EPR6536] to Histone H1.0 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Histone H1.0 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P07305

  • Positive control

    • Human fetal kidney and BxPC3 cell lysates; Human colon tissue; BxPC3 cells.
  • General notes

    Ab203946 is the carrier-free version of ab134914. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab203946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
    • Sequence similarities

      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-17 in RNA edited version.
    • Cellular localization

      Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • H1 histone family member 0 antibody
      • H1(0) antibody
      • H10 antibody
      • H10_HUMAN antibody
      • h1f0 antibody
      • H1FV antibody
      • Histone H1'' antibody
      • Histone H1(0) antibody
      • Histone H1.0 antibody
      • Histone H10 antibody
      • Histone H5 antibody
      • MGC5241 antibody
      • N-terminally processed antibody
      see all

    References

    ab203946 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab203946.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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