Recombinant
RabMAb

Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Submit an Abreview Q&A (2)Specific References (3)

Overview

  • Product name
    Anti-Histone H1.0 antibody [EPR6537]
    See all Histone H1.0 primary antibodies
  • Description
    Rabbit monoclonal [EPR6537] to Histone H1.0
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Histone H1.0 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P07305

  • Positive control
    • WB: BxPC3 and fetal Human lung lysates IHC-P: Human kidney tissue ICC/IF: A431 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/400. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-17 in RNA edited version.
  • Cellular localization
    Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • Database links
    • Alternative names
      • H1 histone family member 0 antibody
      • H1(0) antibody
      • H10 antibody
      • H10_HUMAN antibody
      • h1f0 antibody
      • H1FV antibody
      • Histone H1'' antibody
      • Histone H1(0) antibody
      • Histone H1.0 antibody
      • Histone H10 antibody
      • Histone H5 antibody
      • MGC5241 antibody
      • N-terminally processed antibody
      see all

    Images

    • Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 10 µg (purified)

      Lane 1 : human fetal lung lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : A431 lysate
      Lane 4 : human fetal kidney lysate

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 32 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

      Immunofluorescence staining of BxPC-3 cells with purified ab125027 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab125027 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemical staining of paraffin embedded rat kidney with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemical staining of paraffin embedded mouse liver with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemical staining of paraffin embedded human pancreas with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution (unpurified)

      Lane 1 : BxPC3 lysates
      Lane 2 : Human fetal lung lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 21 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

      Unpurified ab125027, at a 1/100 dilution, staining Histone H1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

      Unpurified ab125027, at a 1/100 dilution, staining Histone H1 in A431 cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Brighton PJ  et al. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium. Elife 6:N/A (2017). Read more (PubMed: 29227245) »
    • Sato M  et al. LW6, a hypoxia-inducible factor 1 inhibitor, selectively induces apoptosis in hypoxic cells through depolarization of mitochondria in A549 human lung cancer cells. Mol Med Rep 12:3462-8 (2015). WB ; Human . Read more (PubMed: 26017562) »

    See all 3 Publications for this product

    Publishing research using ab125027? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Thank you for your response. Histone H1.2, 1.3 or 1.4 are about 21-22KD. Which of your antibody recognize these three isoforms, especially H1.4?
    I also notice that you have Anti-Histone H1 (tri methyl K25) antibody (ab17347) which detects H1.4K25....

    Read More

    Thank you for contacting us.

    H1.2 specific antibodies,
    ab4086; https://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
    ab17677; https://www.abcam.com/Histone-H1-...

    Read More

    Would you please help to verify the following questions regarding your
    anti-histone H1 anti bodies:
    Anti-Histone H1 antibody (ab87556): Detects a ˜32KD band;
    Anti-Histone H1 antibody (ab83058) : Detects a ˜24KD band;
    Anti-Histone H1...

    Read More

    Thank you for contacting us.

    ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

    ab83058; the sequence used is from P07305 http://...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up