Recombinant Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free
See all Histone H1.0 primary antibodies -
Description
Rabbit monoclonal [EPR6537] to Histone H1.0 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Histone H1.0 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07305 -
Positive control
- WB: A431 cell lysate.
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General notes
ab248104 is the carrier-free version of ab125027. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248104 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6537 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab248104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsPhosphorylated on Ser-17 in RNA edited version. -
Cellular localization
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 3005 Human
- Omim: 142708 Human
- SwissProt: P07305 Human
- Unigene: 745024 Human
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Alternative names
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
Images
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![Western blot - Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)](https://www.abcam.com/ps/products/248/ab248104/Images/ab248104-380879-anti-histone-h10-antibody-epr6537-bsa-and-azide-free-western-blot-a431-wt-a431-h1f0-knockout-hela-mcf7.jpg)
Western blot - Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : H1F0 knockout A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab125027).
Lanes 1 - 2: Merged signal (red and green). Green - ab125027 observed at 33 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab125027 was shown to react with H1F0 in A431 wild-type cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. A431 wild-type and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab125027 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Datasheets and documents
References
ab248104 has not yet been referenced specifically in any publications.
