Recombinant Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)
![Western blot - Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)](https://www.abcam.com/ps/products/248/ab248104/Images/ab248104-380879-anti-histone-h10-antibody-epr6537-bsa-and-azide-free-western-blot-a431-wt-a431-h1f0-knockout-hela-mcf7.jpg "Western blot - Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6537] to Histone H1.0 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free
See all Histone H1.0 primary antibodies -
Description
Rabbit monoclonal [EPR6537] to Histone H1.0 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate.
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General notes
ab248104 is the carrier-free version of ab125027.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6537 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
|
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
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| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. |
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsPhosphorylated on Ser-17 in RNA edited version. -
Cellular localization
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 3005 Human
- Omim: 142708 Human
- SwissProt: P07305 Human
- Unigene: 745024 Human
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Alternative names
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
Images
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Western blot - Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : H1F0 knockout A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab125027).
Lanes 1 - 2: Merged signal (red and green). Green - ab125027 observed at 33 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab125027 was shown to react with H1F0 in A431 wild-type cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. A431 wild-type and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab125027 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)](https://www.abcam.com/ps/products/248/ab248104/Images/ab248104-2-benefits-of-recombinant-antibodies.png)
Anti-Histone H1.0 antibody [EPR6537] - BSA and Azide free (ab248104)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248104 has not yet been referenced specifically in any publications.
