Anti-Histone H1.1 antibody (ab17584)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Histone H1.1 antibody
See all Histone H1.1 primary antibodies -
DescriptionRabbit polyclonal to Histone H1.1
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Host speciesRabbit
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SpecificityReacts with recombinant Human H1.1, not with other Human H1 isoforms or core histones. Does not react with histone H1 acid extracted from cell-lines such as breast cancer cell line T47D, because H1.1 is not expressed in this cell line. H1.1 is not expressed in many cell lines, as has been previously reported (Meergans et al, 1997)
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
SAAPEKPLAGKKAKKPAKAAAASKKKPA-C
, corresponding to amino acids 12-39 of Human Histone H1.1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line. |
Target
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RelevanceHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0/1/2/3/4/5.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 3024 Human
- NCBI: NP_005316 Human
- SwissProt: Q02539 Human
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Alternative names
- H1 histone family member 1 antibody
- H1.1 antibody
- H1F1 antibody
see all
Images
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Western blot - Anti-Histone H1.1 antibody (ab17584)This image is courtesy of Dr Albert Jordan, Center for Genomic Regulation (CRG).Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.Notice that H1.1 is not present/detected in the chromatin preparation.Recombinant isoforms courtesy of Dr. Nicole HappelRabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.Notice that H
Protocols
Datasheets and documents
References
This product has been referenced in:
- Johann DJ et al. Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. J Proteome Res 8:2310-8 (2009). WB ; Human . Read more (PubMed: 19284784) »
- Happel N et al. H1 histone subtype constitution and phosphorylation state of the ageing cell system of human peripheral blood lymphocytes. Exp Gerontol 43:184-99 (2008). Read more (PubMed: 18234461) »
