Overview

  • Product name
    Anti-Histone H1.1 antibody
    See all Histone H1.1 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.1
  • Host species
    Rabbit
  • Specificity
    Reacts with recombinant Human H1.1, not with other Human H1 isoforms or core histones. Does not react with histone H1 acid extracted from cell-lines such as breast cancer cell line T47D, because H1.1 is not expressed in this cell line. H1.1 is not expressed in many cell lines, as has been previously reported (Meergans et al, 1997)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SAAPEKPLAGKKAKKPAKAAAASKKKPA-C

    , corresponding to amino acids 12-39 of Human Histone H1.1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab17584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line.

Target

  • Relevance
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0/1/2/3/4/5.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • H1 histone family member 1 antibody
      • H1.1 antibody
      • H1F1 antibody
      • HIST1 antibody
      • HIST1H1A antibody
      • Histone 1 H1a antibody
      • Histone cluster 1 H1a antibody
      see all

    Images

    • Western blot - Anti-Histone H1.1 antibody (ab17584)
      Western blot - Anti-Histone H1.1 antibody (ab17584)This image is courtesy of Dr Albert Jordan, Center for Genomic Regulation (CRG).
      Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.
       
      Notice that H1.1 is not present/detected in the chromatin  preparation.
       
      Recombinant isoforms courtesy of Dr. Nicole Happel
      Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.
       
      Notice that H

    References

    This product has been referenced in:
    • Johann DJ  et al. Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. J Proteome Res 8:2310-8 (2009). WB ; Human . Read more (PubMed: 19284784) »
    • Happel N  et al. H1 histone subtype constitution and phosphorylation state of the ageing cell system of human peripheral blood lymphocytes. Exp Gerontol 43:184-99 (2008). Read more (PubMed: 18234461) »

    See all 3 Publications for this product

    Publishing research using ab17584? Please let us know so that we can cite the reference in this datasheet.

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    Western blot

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    Username

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    Verified customer

    Submitted Jun 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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