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Epigenetics and Nuclear Signaling Histones Variants
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Anti-Histone H1.1 antibody (ab17584)

  • Datasheet
Reviews (1) Submit a question References (3)

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Western blot - Anti-Histone H1.1 antibody (ab17584)

    Key features and details

    • Rabbit polyclonal to Histone H1.1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-Histone H1.5 antibody - ChIP Grade (ab18208)
    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Histone H1.1 antibody
      See all Histone H1.1 primary antibodies
    • Description

      Rabbit polyclonal to Histone H1.1
    • Host species

      Rabbit
    • Specificity

      Reacts with recombinant Human H1.1, not with other Human H1 isoforms or core histones. Does not react with histone H1 acid extracted from cell-lines such as breast cancer cell line T47D, because H1.1 is not expressed in this cell line. H1.1 is not expressed in many cell lines, as has been previously reported (Meergans et al, 1997)
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide:

      SAAPEKPLAGKKAKKPAKAAAASKKKPA-C

      , corresponding to amino acids 12-39 of Human Histone H1.1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Histones
      • Variants
      • Epigenetics and Nuclear Signaling
      • Histones
      • H1
      • Unmodified

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17584 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line.
    Notes
    WB
    1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line.

    Target

    • Relevance

      Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0/1/2/3/4/5.
    • Cellular localization

      Nuclear
    • Database links

      • Entrez Gene: 3024 Human
      • NCBI: NP_005316 Human
      • SwissProt: Q02539 Human
      • Alternative names

        • H1 histone family member 1 antibody
        • H1.1 antibody
        • H1F1 antibody
        • HIST1 antibody
        • HIST1H1A antibody
        • Histone 1 H1a antibody
        • Histone cluster 1 H1a antibody
        see all

      Images

      • Western blot - Anti-Histone H1.1 antibody (ab17584)
        Western blot - Anti-Histone H1.1 antibody (ab17584)This image is courtesy of Dr Albert Jordan, Center for Genomic Regulation (CRG).
        Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.
         
        Notice that H1.1 is not present/detected in the chromatin  preparation.
         
        Recombinant isoforms courtesy of Dr. Nicole Happel
        Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.
         
        Notice that H

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (3)

      Publishing research using ab17584? Please let us know so that we can cite the reference in this datasheet.

      ab17584 has been referenced in 3 publications.

      • Johann DJ  et al. Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. J Proteome Res 8:2310-8 (2009). WB ; Human . PubMed: 19284784
      • Happel N  et al. H1 histone subtype constitution and phosphorylation state of the ageing cell system of human peripheral blood lymphocytes. Exp Gerontol 43:184-99 (2008). PubMed: 18234461
      • Sancho M  et al. Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth. PLoS Genet 4:e1000227 (2008). WB ; Human . PubMed: 18927631

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot

      Average
      Abreviews
      Abreviews
      Application
      Western blot
      Loading amount
      40 µg
      Gel Running Conditions
      Non-reduced Denaturing (10)
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Specification
      HeLa cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jun 07 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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