Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody
See all Histone H1.2 primary antibodies -
Description
Rabbit polyclonal to Histone H1.2 (2-hydroxyisobutyryl K158) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, ICC/IF, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H1.2 (2-hydroxyisobutyryl K158).
Database link: P16403 -
Positive control
- WB: HeLa, HEK-293, A549 and K562 whole cell lysates, treated with 30mM sodium butyrate for 4 hours. ICC: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Predicted molecular weight: 21 kDa. | |
| ChIP | Use 5µg for 106 cells. | |
| ICC/IF | 1/10 - 1/100. | |
| ICC | 1/10. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3006 Human
- Omim: 142710 Human
- SwissProt: P16403 Human
- Unigene: 7644 Human
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Alternative names
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
see all
Images
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Western blot - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)All lanes : Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
Lane 7 : A549 whole cell lysate, untreated (-)
Lane 8 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa -
Immunocytochemistry - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H1.2 (2-hydroxyisobutyryl K158) with ab242273 at 1/10 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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ChIP - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)HeLa (human epithelial cell line from cervix adenocarcinoma; (106, treated with 30mM sodium butyrate for 4 hours) cells were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab242273 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
References
ab242273 has not yet been referenced specifically in any publications.
