Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)
Key features and details
- Rabbit polyclonal to Histone H1.2 (2-hydroxyisobutyryl K158)
- Suitable for: WB, ChIP, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody
See all Histone H1.2 primary antibodies -
Description
Rabbit polyclonal to Histone H1.2 (2-hydroxyisobutyryl K158) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H1.2 (2-hydroxyisobutyryl K158).
Database link: P16403 -
Positive control
- WB: HeLa, HEK-293, A549 and K562 whole cell lysates, treated with 30mM sodium butyrate for 4 hours. ICC: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Predicted molecular weight: 21 kDa. | |
| ChIP | Use 5µg for 106 cells. | |
| ICC | 1/10. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3006 Human
- Omim: 142710 Human
- SwissProt: P16403 Human
- Unigene: 7644 Human
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Alternative names
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
see all
Images
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Western blot - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)All lanes : Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
Lane 7 : A549 whole cell lysate, untreated (-)
Lane 8 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa -
Immunocytochemistry - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H1.2 (2-hydroxyisobutyryl K158) with ab242273 at 1/10 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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ChIP - Anti-Histone H1.2 (2-hydroxyisobutyryl K158) antibody (ab242273)HeLa (human epithelial cell line from cervix adenocarcinoma; (106, treated with 30mM sodium butyrate for 4 hours) cells were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab242273 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab242273 has not yet been referenced specifically in any publications.
