• Product name
    Anti-Histone H1.2 antibody
    See all Histone H1.2 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1.2.

  • Positive control
    • This antibody gave a positive signal in Human H1.2 Recombinant Protein.



Our Abpromise guarantee covers the use of ab17677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 2 antibody
    • H1.a antibody
    • H12_HUMAN antibody
    • H1F2 antibody
    • H1s-1 antibody
    • HIST1H1C antibody
    • Histone 1 H1c antibody
    • Histone cluster 1 H1c antibody
    • Histone H1.2 antibody
    • Histone H1c antibody
    • Histone H1d antibody
    • Histone H1s-1 antibody
    • MGC3992 antibody
    see all


  • Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H1.2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 35kDa; Histone H1.2

  • All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution

    Lane 1 : Recombinant histone H1
    Lane 2 : Recombinant histone H1.1
    Lane 3 : Recombinant histone H1.2
    Lane 4 : Recombinant histone H1.3
    Lane 5 : Recombinant histone H1.4
    Lane 6 : Recombinant histone H1.5

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Image courtesy of Human Protein Atlas

    ab17677 staining histone H1.2 in human testis, showing a distinct and strong staining pattern in ductus seminiferus and leydig cells. Paraffin embedded human skin tissue was incubated with ab17677 (1/1200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab17677 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab17677 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17677, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

  • All lanes : Anti-Histone H1.2 antibody (ab17677) at 1 µg/ml

    Lane 1 : Histone H1 Recombinant Protein
    Lane 2 : Histone H1.2 (Human) - Recombinant Protein
    Lane 3 : Histone H1 Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml
    Lane 4 : Histone H1.2 (Human) - Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml

    Lysates/proteins at 0.1 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 12 minutes


This product has been referenced in:
  • Izquierdo-Bouldstridge A  et al. Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats. Nucleic Acids Res 45:11622-11642 (2017). Read more (PubMed: 28977426) »
  • Gómez-Herreros F  et al. TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription. Nat Commun 8:233 (2017). WB ; Human . Read more (PubMed: 28794467) »

See all 19 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and sharing the results you have observed with the anti-Histone H1.2 antibody (ab17677).
Although the Histone H1.2 is expected to be observed at ˜29 kDa, this size can vary depending on the post-translational modificati...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (A549 lung carcinoma)
A549 lung carcinoma
Yes - 1% TX-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (Human B chronic lymphocytic leukaemia cells)
Loading amount
18 µg
Human B chronic lymphocytic leukaemia cells
Gel Running Conditions
Reduced Denaturing (12 % Tris Glycine Gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 14 2008


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