
Recombinant Anti-Histone H1.2 antibody [EPR12690] (ab181973)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Histone H1.2 antibody [EPR12690]
See all Histone H1.2 primary antibodies -
Description
Rabbit monoclonal [EPR12690] to Histone H1.2 -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Histone H1.2 aa 1-100. The exact sequence is proprietary.
Database link: P16403 -
Positive control
- HeLa , A375, 293 and MCF7 cell lysates; Human ovarian carcinoma tissue; Mouse muscle tissue; MCF7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12690 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP | Use at an assay dependent concentration. | |
WB | 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 21 kDa). | |
IHC-P | 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
ICC/IF | 1/250. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3006 Human
- Entrez Gene: 50708 Mouse
- Entrez Gene: 201097 Rat
- Omim: 142710 Human
- SwissProt: P16403 Human
- SwissProt: P15864 Mouse
- SwissProt: P15865 Rat
- Unigene: 7644 Human
see all -
Alternative names
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
see all
Images
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All lanes : Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted? -
ChIP - Anti-Histone H1.2 antibody [EPR12690] (ab181973)This image is courtesy of an anonymous Abreview
ChIP analysis using ab181973 binding Histone H1.2 in mouse primary hepatocytes. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with the primary antibody (1/100) for 20 hours at 4°C. Protein binding was detected using real-time PCR.
Negative Control: IgG. -
ab181973 staining Histone H1.2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
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Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/2000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Histone H1.2 with ab181973 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green), Dapi staining (blue).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hangas A et al. Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2. Nucleic Acids Res 46:9625-9636 (2018). Read more (PubMed: 30169847) »