Anti-Histone H1.2 antibody [EPR12690] (ab181973)

ChIP analysis using ab181973 binding Histone H1.2 in mouse primary hepatocytes. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with the primary antibody (1/100) for 20 hours at 4°C. Protein binding was detected using real-time PCR.
Negative Control: IgG.

See Abreview

ab181973 staining Histone H1.2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control: PBS only

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Histone H1.2 with ab181973 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green), Dapi staining (blue).