Recombinant
RabMAb

Recombinant Anti-Histone H1.2 antibody [EPR12690] (ab181973)

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Overview

  • Product name

    Anti-Histone H1.2 antibody [EPR12690]
    See all Histone H1.2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12690] to Histone H1.2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Histone H1.2 aa 1-100. The exact sequence is proprietary.
    Database link: P16403

  • Positive control

    • HeLa , A375, 293 and MCF7 cell lysates; Human ovarian carcinoma tissue; Mouse muscle tissue; MCF7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Target

Images

  • Western blot - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Western blot - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    All lanes : Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?

  • ChIP - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    ChIP - Anti-Histone H1.2 antibody [EPR12690] (ab181973)This image is courtesy of an anonymous Abreview

    ChIP analysis using ab181973 binding Histone H1.2 in mouse primary hepatocytes. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with the primary antibody (1/100) for 20 hours at 4°C. Protein binding was detected using real-time PCR.
    Negative Control: IgG.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody [EPR12690] (ab181973)

    ab181973 staining Histone H1.2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • Western blot - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Western blot - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Anti-Histone H1.2 antibody [EPR12690] (ab181973) at 1/2000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.2 antibody [EPR12690] (ab181973)

    Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labeling Histone H1.2 with ab181973 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody [EPR12690] (ab181973)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody [EPR12690] (ab181973)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Histone H1.2 with ab181973 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green), Dapi staining (blue).

References

This product has been referenced in:

  • Hangas A  et al. Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2. Nucleic Acids Res 46:9625-9636 (2018). Read more (PubMed: 30169847) »
See 1 Publication for this product

Publishing research using ab181973? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H1.2 antibody [EPR12690]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (fibroblasts/embryos)
Permeabilization
Yes - Triton 100X - 0.5%
Specification
fibroblasts/embryos
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Dr. Vilceu Bordignon

Verified customer

Submitted Dec 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H1.2 antibody [EPR12690]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
3%BSA+0.1% Triton X100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Sample
Mouse Cell (primary hepatoctyes)
Specification
primary hepatoctyes
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 26 2014

Western blot abreview for Anti-Histone H1.2 antibody [EPR12690]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Mar 11 2014

Immunoprecipitation abreview for Anti-Histone H1.2 antibody [EPR12690]

 Poor
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Total protein in input
25 µg
Read More

Abcam user community

Verified customer

Submitted Mar 05 2014

ChIP abreview for Anti-Histone H1.2 antibody [EPR12690]

 Average
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
no
Read More

Abcam user community

Verified customer

Submitted Mar 03 2014

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