Product nameAnti-Histone H1.2 (formyl K109) antibody
See all Histone H1.2 primary antibodies
DescriptionRabbit polyclonal to Histone H1.2 (formyl K109)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Histone H1.2 (formyl K109).
Database link: P16403
- WB: K562, treated with 30mM sodium butyrate for 4hr, whole cell lysate. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). ICC: HeLa cells (treated with 30mM sodium butyrate for 4hr).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 21 kDa.|
|ICC/IF||1/10 - 1/100.|
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
All lanes : Anti-Histone H1.2 (formyl K109) antibody (ab241057) at 1/400 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : K562 whole cell lysate, untreated (-)
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H1.2 (formyl K122) using ab241057 at 1/20 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-congugated Goat Anti-Rabbit IgG(H+L).
HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H1.2 (formyl K109) using ab241057 at 1/20 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ab241057 has not yet been referenced specifically in any publications.