Overview

  • Product name

    Anti-Histone H1.2 (formyl K109) antibody
    See all Histone H1.2 primary antibodies
  • Description

    Rabbit polyclonal to Histone H1.2 (formyl K109)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Histone H1.2 (formyl K109).
    Database link: P16403

  • Positive control

    • WB: K562, treated with 30mM sodium butyrate for 4hr, whole cell lysate. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). ICC: HeLa cells (treated with 30mM sodium butyrate for 4hr).

Properties

Applications

Our Abpromise guarantee covers the use of ab241057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/10 - 1/100.

Target

  • Function

    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H1 histone family member 2 antibody
    • H1.a antibody
    • H12_HUMAN antibody
    • H1F2 antibody
    • H1s-1 antibody
    • HIST1H1C antibody
    • Histone 1 H1c antibody
    • Histone cluster 1 H1c antibody
    • Histone H1.2 antibody
    • Histone H1c antibody
    • Histone H1d antibody
    • Histone H1s-1 antibody
    • MGC3992 antibody
    see all

Images

  • All lanes : Anti-Histone H1.2 (formyl K109) antibody (ab241057) at 1/400 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
    Lane 2 : K562 whole cell lysate, untreated (-)

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 21 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H1.2 (formyl K122) using ab241057 at 1/20 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-congugated Goat Anti-Rabbit IgG(H+L).

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H1.2 (formyl K109) using ab241057 at 1/20 dilution in ICC.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab241057 has not yet been referenced specifically in any publications.

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