Anti-Histone H1.2 (formyl K96) antibody (ab241251)
Key features and details
- Rabbit polyclonal to Histone H1.2 (formyl K96)
- Suitable for: ICC, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.2 (formyl K96) antibody
See all Histone H1.2 primary antibodies -
Description
Rabbit polyclonal to Histone H1.2 (formyl K96) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H1.2 (formyl K96).
Database link: P16403 -
Positive control
- WB: HeLa, HEK-293T, HepG2 and A549, treated with 30mM sodium butyrate for 4hr, whole cell lysates. ICC/IF: HeLa cells. ICC: HeLa cells (treated with 30mM sodium butyrate for 4hr).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/1 - 1/10. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 21 kDa. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3006 Human
- Omim: 142710 Human
- SwissProt: P16403 Human
- Unigene: 7644 Human
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Alternative names
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
see all
Images
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Western blot - Anti-Histone H1.2 (formyl K96) antibody (ab241251)All lanes : Anti-Histone H1.2 (formyl K96) antibody (ab241251) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 5 : HeLa whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
Lane 7 : HepG2 whole cell lysate, untreated (-)
Lane 8 : A549 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 (formyl K96) antibody (ab241251)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H1.2 (formyl K96) (green) using ab241251 at 1/50 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
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Immunocytochemistry - Anti-Histone H1.2 (formyl K96) antibody (ab241251)HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 30mM sodium butyrate for 4hr) stained for Histone H1.2 (formyl K96) using ab241251 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab241251 has not yet been referenced specifically in any publications.
