Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab24174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50.
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). H1.3 is present in many human cell lines. Cross-reacts with bands of approx. 52kDa and 45kDa in nuclear extracts of T47D cell line.

Target

  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 3 antibody
    • H1.3 antibody
    • H13_HUMAN antibody
    • H1F3 antibody
    • HIST1 H1D antibody
    • HIST1H1D antibody
    • Histone 1 H1d antibody
    • Histone cluster 1 H1d antibody
    • Histone H1.3 antibody
    • Histone H1c antibody
    • MGC138176 antibody
    see all

Images

  • Rabbit polyclonal to Histone H1.3 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line. Notice that extra bands are present/detected in the chromatin preparation.
  • Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab24174 (1/200) for Histone H1.3 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
  • IHC image of ab24174 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24174, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Li Z  et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res 28:756-770 (2018). Read more (PubMed: 29844578) »
  • Izquierdo-Bouldstridge A  et al. Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats. Nucleic Acids Res 45:11622-11642 (2017). Read more (PubMed: 28977426) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (12.5)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted May 28 2013

Question
Answer

Unfortunately the peptide is not available for purchase.

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Answer

Yes you are correct; To clarify, H1.3 corresponds to H1c and HIST1H1D. H1.4 corresponds to H1b or HIST1H1E.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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