Product nameAnti-Histone H1.3 antibody [EPR12683]
See all Histone H1.3 primary antibodies
DescriptionRabbit monoclonal [EPR12683] to Histone H1.3
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Histone H1.3 aa 1-100. The exact sequence is proprietary.
Database link: P16402
- T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab183736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member 3 antibody
- H1.3 antibody
- H13_HUMAN antibody
All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Blocking buffer: 5% NFDM/TBST
Flow cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab183736 has not yet been referenced specifically in any publications.