Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12683] to Histone H1.3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-Histone H1.3 antibody [EPR12683]
See all Histone H1.3 primary antibodies
DescriptionRabbit monoclonal [EPR12683] to Histone H1.3
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Histone H1.3 aa 1-100. The exact sequence is proprietary.
Database link: P16402
- T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab183736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member 3 antibody
- H1.3 antibody
- H13_HUMAN antibody
All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Blocking buffer: 5% NFDM/TBST
Flow cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab183736 has not yet been referenced specifically in any publications.