Anti-Histone H1.4 (phospho T18) antibody (ab203624)
Key features and details
- Rabbit polyclonal to Histone H1.4 (phospho T18)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.4 (phospho T18) antibody -
Description
Rabbit polyclonal to Histone H1.4 (phospho T18) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Histone H1.4 aa 1-100 (phospho T18) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P10412 -
Positive control
- Mouse liver and cerebrum lysates, rat brain tissue, human colon carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe use at 1/50 - 1/200 |
Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 22 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe use at 1/50 - 1/200 |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 50709 Mouse
- SwissProt: P43274 Mouse
- Unigene: 170587 Mouse
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Alternative names
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
see all
Images
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Immunohistochemical analysis of formalin fixed paraffin embedded rat brain labeling Histone H1.4 (phospho T18) with ab203624 at 1/200 followed by conjugation to the secondary antibody and DAB staining
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All lanes : Anti-Histone H1.4 (phospho T18) antibody (ab203624) at 1/200 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse cerebrum lysate
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203624 has not yet been referenced specifically in any publications.