Overview

  • Product name

    Anti-Histone H1.4 (phospho T18) antibody
    See all Histone H1.4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H1.4 (phospho T18)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Histone H1.4 aa 2-30 (phospho T18) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    SETAPAAPA APAPAEKTPV KKKARKSAGA


    Database link: P10412

  • Positive control

    • Mouse liver and cerebrum lysates, rat brain tissue, human colon carcinoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe use at 1/50 - 1/200

Target

  • Function

    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications

    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H1 histone family member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1 H1e antibody
    • Histone cluster 1 H1e antibody
    • Histone H1 antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed paraffin embedded rat brain labeling Histone H1.4 (phospho T18) with ab203624 at 1/200 followed by conjugation to the secondary antibody and DAB staining

  • All lanes : Anti-Histone H1.4 (phospho T18) antibody (ab203624) at 1/200 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse cerebrum lysate

    Predicted band size: 22 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

References

ab203624 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203624.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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