Key features and details
- Rabbit polyclonal to Histone H1.4 (phospho T18)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Histone H1.4 (phospho T18) antibody
See all Histone H1.4 primary antibodies
DescriptionRabbit polyclonal to Histone H1.4 (phospho T18)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Mouse liver and cerebrum lysates, rat brain tissue, human colon carcinoma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe use at 1/50 - 1/200
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
Immunohistochemical analysis of formalin fixed paraffin embedded rat brain labeling Histone H1.4 (phospho T18) with ab203624 at 1/200 followed by conjugation to the secondary antibody and DAB staining
All lanes : Anti-Histone H1.4 (phospho T18) antibody (ab203624) at 1/200 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse cerebrum lysate
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab203624 has not yet been referenced specifically in any publications.