Anti-Histone H1.4 (phospho T18) antibody (ab3595)


  • Product name
    Anti-Histone H1.4 (phospho T18) antibody
    See all Histone H1.4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1.4 (phospho T18)
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1, phosphorylated at T18.

    (Peptide available as ab10138.)


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa.
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.


  • Function
    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1 H1e antibody
    • Histone cluster 1 H1e antibody
    • Histone H1 antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    see all


  • Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).

    Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.

    Lanes 1-2    :  wt Histone H1
    Lanes 3-4    :  T146A, T154A, S172A, S187A (wt site at T18)
    Lanes 5-6    :  T18A, T154A, S172A, S187A (wt site at T146)
    Lanes 7-8    :  T18A, T146A, S172A, S187A (wt site at T154)
    Lanes 9-10  :  T18A, T146A, T154A, S187A (wt site at S172)
    Lanes 11-12: 

  • Rabbit polyclonal to Histone H1 (phospho T18) (1/25).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).


This product has been referenced in:
  • Chen Y  et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). Read more (PubMed: 26209608) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab3595. It would be very helpful if you could provide me with some additional details regarding your protocol. This will help us to investigate this matter as quic...

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Thank you for your e-mail. We are very sorry to hear that you are having problem with this antibody. We do not have problem with this antibody and this is the first complaint we have received so far. Certainly, we can send you a new vial as a repl...

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