Key features and details
- Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)
- Suitable for: ELISA
- Isotype: IgG
Product nameAnti-Histone H1.4 (tri methyl K25, phospho S26) antibody
See all Histone H1.4 primary antibodies
Predicted to work with: Mouse, Human
Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
|ELISA||Use at an assay dependent concentration.|
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
This antibody gave a positive result in ELISA against the immunizing peptide, Human Histone H1.4 (tri methyl K25, phospho S26) peptide (blue line).
For the following peptides it gave following results:
the non-modified equivalent peptide (yellow line, ab35918) - weak result,
H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab33231 has been referenced in 1 publication.
- Terme JM et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882