Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.

Overview

  • Product name

    Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody
    See all Histone H1.4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Species reactivity


    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab35913)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA
  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide.
    For the following peptides it gave following results:
    the non-modified equivalent peptide (yellow line, ab35918) - weak result,
    H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
    See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
    • Sequence similarities

      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Post-translational
      modifications

      Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
    • Cellular localization

      Nucleus. Chromosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • H1 histone family member 4 antibody
      • H1.4 antibody
      • H14_HUMAN antibody
      • H1E antibody
      • H1F4 antibody
      • Hist1h1e antibody
      • Histone 1 H1e antibody
      • Histone cluster 1 H1e antibody
      • Histone H1 antibody
      • Histone H1.4 antibody
      • Histone H1B antibody
      • MGC116819 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab35913).
      For the following peptides it gave following results:
      the non-modified equivalent peptide (yellow line, ab35918) - weak result,
      H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

    References

    This product has been referenced in:

    • Terme JM  et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). Read more (PubMed: 24873882) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Purified protein (breast cancer cell line T47D)
    Loading amount
    5 µg
    Specification
    breast cancer cell line T47D
    Treatment
    50ng/ml colcemid for 16h
    Gel Running Conditions
    Non-reduced Denaturing (10 % SDS-PAGE)
    Blocking step
    Odissey blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C

    Dr. Albert Jordan

    Verified customer

    Submitted May 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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