Overview

  • Product name

    Anti-Histone H1oo antibody
  • Description

    Rabbit polyclonal to Histone H1oo
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Histone H1oo (C terminal).
    Database link: Q8VIK3

Properties

Applications

Our Abpromise guarantee covers the use of ab71580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 32 kDa.

Target

  • Function

    May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • Tissue specificity

    Oocyte-specific.
  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H1 histone family member O oocyte specific antibody
    • H1FOO antibody
    • H1FOO_HUMAN antibody
    • Histone H1oo antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    see all

References

This product has been referenced in:

  • Funaya S  et al. Linker histone H1FOO regulates the chromatin structure in mouse zygotes. FEBS Lett 592:2414-2424 (2018). Read more (PubMed: 29963710) »
  • Hou J  et al. Liraglutide, a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage. J Cereb Blood Flow Metab 32:2201-10 (2012). Read more (PubMed: 22968320) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hela, Hep G2)
Specification
Hela, Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 26 2014

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