• Product name

  • Description

    Rabbit polyclonal to Histone H1x
  • Host species

  • Specificity

    ab17729 recognises Histone H1x variant in HeLa nuclear and whole cell extracts (see figures below).
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Histone H1x aa 150 to the C-terminus (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92522
    (Peptide available as ab18053)

  • Positive control

    • HeLa whole cell lysate and HeLa nuclear lysate.



Our Abpromise guarantee covers the use of ab17729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H1 histone family member X antibody
    • H1FX antibody
    • H1X antibody
    • H1X_HUMAN antibody
    • Histone H1x antibody
    • MGC15959 antibody
    • MGC8350 antibody
    see all


  • All lanes : Anti-Histone H1x antibody (ab17729) at 0.5 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : HeLa whole cell extract with Human Histone H1x peptide (ab18053) at 1 µg/ml
    Lane 3 : HeLa nuclear extract

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    ab17729 recognises histone H1.X in HeLa whole cell (lane1) and nuclear extracts (lane3) at 35 kDa, which is efficiently blocked using the immunizing peptide ab18053 (lane2). ab17729 recognises bands at 20 kDa, which is attributed to minor cross-reactivity and may be improved using different blocking conditions.
  • ICC/IF image of ab17729 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab17729, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab17729 staining histone H1.X in male cerebellum, showing a distinct and strong nuclear staining pattern at cells in granular and molecular layers. Paraffin embedded human skin tissue was incubated with ab17729 (1/750 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab17729 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org



This product has been referenced in:

  • Crawford NP  et al. The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284:28660-73 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19710015) »
See 1 Publication for this product

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