Key features and details
- Rabbit polyclonal to Histone H1x
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H1x antibody
See all Histone H1x primary antibodies
DescriptionRabbit polyclonal to Histone H1x
Specificityab17729 recognises Histone H1x variant in HeLa nuclear and whole cell extracts (see figures below).
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- HeLa whole cell lysate and HeLa nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H1 histone family member X antibody
- H1FX antibody
- H1X antibody
All lanes : Anti-Histone H1x antibody (ab17729) at 0.5 µg/ml
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa whole cell extract with Human Histone H1x peptide (ab18053) at 1 µg/ml
Lane 3 : HeLa nuclear extract
Predicted band size: 35 kDa
Observed band size: 35 kDa
ab17729 recognises histone H1.X in HeLa whole cell (lane1) and nuclear extracts (lane3) at 35 kDa, which is efficiently blocked using the immunizing peptide ab18053 (lane2). ab17729 recognises bands at 20 kDa, which is attributed to minor cross-reactivity and may be improved using different blocking conditions.
ICC/IF image of ab17729 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab17729, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas
ab17729 staining histone H1.X in male cerebellum, showing a distinct and strong nuclear staining pattern at cells in granular and molecular layers. Paraffin embedded human skin tissue was incubated with ab17729 (1/750 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6..
ab17729 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ab17729 has been referenced in 1 publication.
- Crawford NP et al. The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284:28660-73 (2009). WB, ICC/IF ; Human . PubMed: 19710015