Product nameAnti-Histone H2A (acetyl K5) antibody - ChIP Grade
See all Histone H2A primary antibodies
DescriptionRabbit polyclonal to Histone H2A (acetyl K5) - ChIP Grade
SpecificityHistone H2A acetylated at lysine 5.
Tested applicationsSuitable for: ChIP, ELISA, IP, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human, Caenorhabditis elegans, Drosophila melanogaster, Mammals, Asian toad
- HL60 cells treated with butyrate. IHC-P: Human normal colon FFPE tissue sections.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
ChIP Related Products
Our Abpromise guarantee covers the use of ab1764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/800 - 1/2000. Detects a band of approximately 20 kDa.
Use 5% BSA in TBST to block.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H2A family.
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2a 615 antibody
- H2A antibody
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Ab1764 at a 1/1000 dilution in Western blot of human Osteosarcoma (U2OS) nuclear cell lysate.
IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab1764 staining Female Embryonic Stem cells differentiated for 7 days by immunocytochemistry. The antibody was used at a dilution of 1/200 and incubated with the cells for 1 hour. Bound antibody was detected with a FITC conjugated Goat anti-Rabbit polyclonal antibody.
DAPI counterstain is shown pseudocoloured as white on the right of the image (b). Centric heterochromatin was underacetylated in differentiated cells (arrowhead in a). A pale staining chromosome was observed at high frequencies; previoulsy shown to be the inactive female X chromosome (arrow in a).
This image is courtesy of an Abreview by Hugh Spotswood submitted on 13 February 2006.
This product has been referenced in:
- Lau AC et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . Read more (PubMed: 27777629) »
- Shirakawa K et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . Read more (PubMed: 27244239) »