Overview

  • Product name

    Anti-Histone H2A (acetyl K5) antibody - N-terminal
    See all Histone H2A primary antibodies
  • Description

    Rabbit polyclonal to Histone H2A (acetyl K5) - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Histone H2A (N terminal) (acetyl K5). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P04908

  • Positive control

    • WB: HeLa treated with 0.4 µM TSA, whole cell lysate. IP: HeLa treated with 0.4 µM TSA, whole cell lysate. ICC/IF: HeLa treated with 0.4 µM TSA cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/10000.
WB 1/1000 - 1/20000. Predicted molecular weight: 14 kDa.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H2A family.
  • Post-translational
    modifications

    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H2a 615 antibody
    • H2A antibody
    • H2A GL101 antibody
    • H2A histone family member A antibody
    • H2A.1 antibody
    • H2A.2 antibody
    • H2A/a antibody
    • H2A/m antibody
    • H2A/O antibody
    • H2A/q antibody
    • H2A1B_HUMAN antibody
    • H2AFA antibody
    • H2AFE antibody
    • H2AFL antibody
    • H2AFM antibody
    • H2AFO antibody
    • H2AFQ antibody
    • HIST1H2AE antibody
    • HIST1H2AJ antibody
    • HIST2H2AA antibody
    • HIST2H2AA3 antibody
    • HIST2H2AB antibody
    • HIST2H2AC antibody
    • Histone 1 H2ae antibody
    • Histone 2 H2aa3 antibody
    • Histone 2 H2ab antibody
    • Histone 2 H2ac antibody
    • Histone H2A type 1 B antibody
    • Histone H2A type 1 C antibody
    • Histone H2A type 1 E antibody
    • Histone H2A type 1 J antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    • MGC74460 antibody
    see all

Images

  • All lanes : Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578) at 1/10000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.012% DMSO for 18 hours, whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.4 µM Trichostatin A for 18 hours, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa



    15% SDS-PAGE gel.

  • Dot blot analysis of Histone H2A (acetyl K5) labeled with ab229578 at 1/500 dilution.

    Lane 1: H2A.2K5un peptide.
    Lane 2: H2A.2K5ac peptide.
    Lane 3: H2BK20un peptide.
    Lane 4: H2BK20ac peptide.
    Lane 5: H2BK5un peptide.
    Lane 6: H2BK5ac peptide.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells (green) untreated (left panels) and treated with 0.4 μM TSA for 18 hours (right panels) stained for Histone H2AK5ac (acetyl Lys5) using ab229578 at 1/500 dilution.

    Nucelar counterstain: Hoechst 33342 (blue).

  • Histone H2A (acetyl K5) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.4 µM TSA for 18 hours, whole cell lysate with 2.5 μg of ab229578. Western blot was performed from the immunoprecipitate using ab229578 at 1/10000 dilution.

    Lane 1: Histone H2A (acetyl K5) expressing HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate 30 μg (Input).
    Lane 2: 2.5 μg of pre-immune rabbit IgG instead of ab229578 in HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate.
    Lane 3: ab229578 IP in HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate.

References

ab229578 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229578.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up