Application
Western blot
Loading amount
0.371 µg
Gel Running Conditions
Reduced Denaturing (20 %)
Sample
Xenopus laevis Recombinant protein (Histones and Nucleosome core particle)
Specification
Histones and Nucleosome core particle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST 0.1 % Sodium azide
Dilution
1/1250
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP DakoCytomation
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 14 and 30 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Histone H2B, other Histones within the nucleosome core particle (H3/H4/H2B)
Abcam user community
Verified customer
Submitted Mar 30 2015