Product nameAnti-Histone H2A antibody - ChIP Grade, purified
See all Histone H2A primary antibodies
DescriptionRabbit polyclonal to Histone H2A - ChIP Grade, purified
Tested applicationsSuitable for: WB, ICC/IF, ChIPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Histone H2A (C terminal) conjugated to keyhole limpet haemocyanin.
- ICC/IF: HeLa cells. WB: HeLa whole cell extract; Histone extract of HeLa cells. ChIP: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin
Concentration information loading...
Our Abpromise guarantee covers the use of ab195322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 14 kDa.|
|ChIP||Use at an assay dependent concentration.
Dilution: 1-2 μg/ChIP
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H2A family.
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
All lanes : Anti-Histone H2A antibody - ChIP Grade, purified (ab195322) at 1/2000 dilution
Lane 1 : HeLa whole cell extract at 25 µg
Lane 2 : Histone extracts of HeLa cells at 15 µg
Lane 3 : Recombinant Histone H2A at 1 µg
Lane 4 : Recombinant Histone H2B at 1 µg
Lane 5 : Recombinant Histone H3 at 1 µg
Lane 6 : Recombinant Histone H4 at 1 µg
Predicted band size: 14 kDa
Immunofluorescent analysis of formaldehyde-fixed HeLa cells labeling Histone H2A with ab195322 at 1/500 dilution (left). The middle panel shows staining of the nuclei with DAPI. A merge of the two images is shown on the right.
ChIP assays using HeLa cells, ab195322 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and EIF4A2 promoters, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. The image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ab195322 has not yet been referenced specifically in any publications.